Summary
An anion channel of sarcoplasmic reticulum vesicle has been incorporated into planar lipid bilayers by means of a fusion method and its basic properties were investigated. Analysis of fusion processes suggested that one SR vesicle contained approximately one anion channel. The conductance of this channel has several substates and shows a flickering behavior. The occupation probability of each substate was voltage dependent, which induced an inward rectification of macroscopic currents. Further, the anion channel was found to have the following properties. (1) The single-channel conductance is about 200 pS at 100mm Cl−. (2) The channel does not select among monovalent anions but SO 2−4 hardly permeates through the channel. (3) SO 2−4 added to thecis side (the side to which SR vesicles were added) inhibits Cl− current competitively in a voltage-dependent manner. (4) An analysis of this voltage dependence suggests that the binding site of SO 2−4 is located at about 36% of the way across the channel from thecis entrance.
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Coronado, R., Miller, C. 1982. Conduction and block by organic cations in a K+-selective channel from sarcoplasmic reticulum incorporated into planar phospholipid bilayers.J. Gen. Physiol. 79:529–547
Coronado, R., Rosenberg, R.L., Miller, C. 1980. Ionic selectivity, saturation, and block in a K+-selective channel from sarcoplasmic reticulum.J. Gen. Physiol. 76:425–446
Gray, P.T.A. Bevan, S., Ritchie, J.M. 1984. High conductance anion-selective channels in rat cultured Schwann cells.Proc. R. Soc. London B 221:395–409
Kasai, M. 1981. Inhibition of the anion permeability of sarcoplasmic reticulum vesicles by some stilbene derivatives.J. Biochem. 89:943–953
Kasai, M., Miyamoto, H. 1976. Depolarization-induced calcium release from sarcoplasmic reticulum fragments II. Release of calcium incorporated without ATP.J. Biochem. (Tokyo) 79:1067–1076
Kasai, M., Nunogaki, K., Nagasaki, K., Tanifuji, M., Sokabe, M. 1985. Ion channels of sarcoplasmic reticulum vesicles and calcium release.In: Structure and Function of Sarcoplasmic Reticulum. S. Fleischer and Y. Tonomura, editors. pp. 537–560. Academic, New York
Kometani, T., Kasai, M. 1978. Ionic permeability of sarcoplasmic reticulum vesicles measured by light scattering method.J. Membrane Biol. 41:295–308
Krouse, M.E., Schneider, G.T., Gage, P.W. 1986. A large anion-selective channel has seven conductance levels.Nature (London) 319:58–60
Labarca, P., Coronado, R., Miller, C. 1980. Thermodynamic and kinetic studies of the gating behavior of a K+-selective channel from the sarcoplasmic reticulum membrane.J. Gen. Physiol. 76:397–424
Martonosi, A.N. 1984. Mechanisms of Ca2+ release from sarcoplasmic reticulum of skeletal muscle.Physiol. Rev. 64:1240–1320
Meissner, G., McKinley, D. 1976. Permeability of sarcoplasmic reticulum membrane. The effect of charged ionic environments on Ca2+ release.J. Membrane Biol. 30:79–98
Miller, C. 1978. Voltage-gated cation conductance channel from fragmented sarcoplasmic reticulum: Steady-state electrical properties.J. Membrane Biol. 40:1–23
Miller, C., Racker, E. 1976. Ca++-induced fusion of fragmented sarcoplasmic reticulum with artificial planar bilayers.J. Membrane Biol. 30:283–300
Miller, C., Rosenberg, R.L. 1979. A voltage-gated cation conductance channel from fragmented sarcoplasmic reticulum. Effects of transition metal ions.Biochemistry 18:1138–1145
Morimoto, T., Kasai, M. 1986. Reconstitution of sarcoplasmic reticulum Ca2+-ATPase vesicles lacking ion channels and demonstration of electrogenicity of Ca2+-pump.J. Biochem. (Tokyo) 99:1071–1080
Mueller, P., Rudin, D.O. 1969. Biomolecular lipid membranes. Techniques of formation, study of electrical properties, and induction of ionic gating phenomena.In: Laboratory Techniques in Membrane Biophysics. H. Passow and R. Stampfli, editors. pp. 141–156. Springer-Verlag, Berlin
Nelson, D.J., Tang, J.M., Palmer, L.G. 1984. Single-channel recordings of apical, membrane chloride conductance in A6 epithelial cells.J. Membrane Biol. 80:81–89
Nunogaki, K., Kasai, M. 1984. Ion permeation through sarcoplasmic reticulum membrane.In: Transmembrane Signaling and Sensation. F. Oosawa, T. Yoshioka and H. Hayashi, editors. pp. 217–227. Japan Scientific Societies Press, Tokyo
Woodhull, A.M. 1973. Ionic blockage of sodium channels in nerve.J. Gen. Physiol. 61:687–708
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Tanifuji, M., Sokabe, M. & Kasai, M. An anion channel of sarcoplasmic reticulum incorporated into planar lipid bilayers: Single-channel behavior and conductance properties. J. Membrain Biol. 99, 103–111 (1987). https://doi.org/10.1007/BF01871230
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DOI: https://doi.org/10.1007/BF01871230