The Journal of Membrane Biology

, Volume 98, Issue 3, pp 223–236 | Cite as

Collagen receptors mediate early events in the attachment of epithelial (MDCK) cells

  • Pedro J. I. Salas
  • Dora E. Vega-Salas
  • Enrique Rodriguez-Boulan
Articles

Summary

Madin-Darby canine kidney (MDCK) cells kept in suspension culture for 12–15 hr displayed high-affinity binding sites for125I-lathyritic (soluble) collagen (120,000/cell,K D =30nm) and preferred collagens types I and IV over laminin or fibronectin as substrates during the first hour of attachment. On the other hand, after 4 hr, attachment to all four substrates was equally efficient. Upon challenge with a collagen substrate, the high-affinity sites were rapidly recruited on it (T1/2=6 min). Their occupancy by soluble collagen triggered the exocytosis of a second large population of low-affinity collagen binding sites that included laminin and seems to be involved in a second cell-attachment mechanism. These results are compatible with a twostep model of MDCK cell attachment to the substrate: first, via high-affinity collagen binding sites, and second, via laminin of cellular origin.

Key Words

epithelia MDCK cells cell-substrate interaction collagen receptor epithelial polarity laminin 

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Copyright information

© Springer-Verlag New York Inc. 1987

Authors and Affiliations

  • Pedro J. I. Salas
    • 1
  • Dora E. Vega-Salas
    • 1
  • Enrique Rodriguez-Boulan
    • 1
  1. 1.Department of Cell Biology and AnatomyCornell University Medical CollegeNew York

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