Skip to main content
SpringerLink
Log in

The role of electrogenic pump inChara corallina

  • Articles
  • Published:
The Journal of Membrane Biology Aims and scope Submit manuscript

Summary

The conductance,G, and the electromotive force,E, of theChara membrane were determined accurately by using the current-clamp technique. The measurements at the final steady state of inhibitor poisoning give the conductance,g 1, and the electromotive force,E 1, of the passive ion conducting pathways. By knowing these values the conductance,g 2, and the electromotive force,E 2, of the electrogenic pump can be calculated from the measuredG andE at each time during the progress of inhibitor poisoning. The local closed circuit current,i, which usually causes a hyperpolarization across the passive conducting pathways, can be calculated by usingg 1,g 2,E 1 andE 2 thus determined. The values ofg 2 andi decrease monotonically to zero with the progress of poisoning, whileE 2 approachesE 1 asymptotically after a transient hyperpolarization. During excitationi increases markedly. Such an increased inward current through the passive conducting pathways may help in accelerating the inactivation of the excitatory mechanism.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Cole, K.S. 1968. Membranes, Ions and Impulses. II and III. University California Press. Berkeley-Los Angeles

    Google Scholar 

  • Cole, K.S., Kishimoto, U. 1962. Platinized silver chloride electrode.Science 136:381

    PubMed  Google Scholar 

  • Cole, K.S., Moore, J.W. 1960. Ionic current measurements in the squid giant axon membrane.J. Gen. Physiol. 44:123

    PubMed  Google Scholar 

  • Gould, J.M. 1976. Inhibition by triphenyltin chloride of a tightly bound membrane component involved in photophosphorylation.J. Biochem. 62:567

    Google Scholar 

  • Gradmann, D. 1975. Analog circuit of theAcetabularia membrane.J. Membrane Biol. 25:183

    Google Scholar 

  • Gradmann, D., Hansen, U.-P., Long, W.S., Slayman, C.L., Warncke, J. 1978. Current-voltage relationships for the plasma membrane and its principal electrogenic pump inNeurospora crassa: I. Steady-state conditions.J. Membrane Biol. 39:333

    Google Scholar 

  • Hopfer, U., Lehninger, A.L., Thompson, T.E. 1968. Protonic conductance across lipid bilayer membranes induced by uncoupling agents for oxidative phosphorylation.Proc. Nat. Acad. Sci. USA 59:484

    PubMed  Google Scholar 

  • Keifer, D.W., Spanswick, R.W. 1978. Activity of the electrogenic pump inChara corallina as inferred from measurements of the membrane potential, conductance and potassium permeability.Plant Physiol. 62:653

    Google Scholar 

  • Keifer, D.W., Spanswick, R.M. 1979. Correlation of adenosine triphosphate levels inChara corallina with the activity of the electrogenic pump.Plant Physiol. 64:165

    Google Scholar 

  • Kitasato, H. 1968. The influence of H+ on the membrane potential and ion fluxes ofNitella.J. Gen. Physiol. 52:60

    PubMed  Google Scholar 

  • MacRobbie, E.A.C. 1970. The active transport of ions in plant cells.Q. Rev. Biophys. 3:251

    Google Scholar 

  • Rapoport, S.I. 1970. The sodium-potassium exchange pump: Relation of metabolism to electrical properties of the cell. I. Theory.Biophys. J. 10:246

    PubMed  Google Scholar 

  • Richard, J.L., Hope, A.B. 1974. The role of protons in determining membrane electrical characteristics inChara corallina.J. Membrane Biol. 16:121

    Google Scholar 

  • Saito, K., Senda, M. 1974. The electrogenic ion pump revealed by the external pH effect on the membrane potential ofNitella. Influences of external ions and electrical current on the pH effect.Plant Cell Physiol. 15:1007

    Google Scholar 

  • Shimmen, T., Tazawa, M. 1977. Control of membrane potential and excitability ofChara cells with ATP and Mg2+.J. Membrane Biol. 37:167

    Google Scholar 

  • Slayman, C.L. 1965a. Electrical properties ofNeurospora crassa: Effects of external cations on the intracellular potential.J. Gen. Physiol. 49:69

    PubMed  Google Scholar 

  • Slayman, C.L. 1965b. Electrical properties ofNeurospora crassa: Respiration and the intracellular potential.J. Gen. Physiol. 49:93

    PubMed  Google Scholar 

  • Slayman, C.L., Long, W.S., Lu, C.Y.-H. 1973. The relationship between ATP and an electrogenic pump in the plasma membrane ofNeurospora crassa.J. Membrane Biol. 14:305

    Google Scholar 

  • Spanswick, R.M. 1972. Evidence for an electrogenic ion pump inNitella translucens. I. The effects of pH, K+, Na+, light and temperature on the membrane potential and resistance.Biochim. Biophys. Acta 288:73

    PubMed  Google Scholar 

  • Stockdale, M., Dawson, A.P., Selwyn, M.J. 1970. Effects of trialkyltin and triphenyltin compounds on mitochondrial respiration.Eur. J. Biochem. 15:342

    PubMed  Google Scholar 

  • Ussing, H.H., Zerahn, K. 1951. Active transport of sodium as the source of electrical current in the short-circuited isolated frog skin.Acta Physiol. Scand. 23:110

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biology, College of General Education, 560, Toyonaka, Japan

    U. Kishimoto, N. Kami-ike & Y. Takeuchi

  2. Department of Bioengineering, Faculty of Engineering Science, Osaka University, 560, Toyonaka, Japan

    U. Kishimoto, N. Kami-ike & Y. Takeuchi

Authors
  1. U. Kishimoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. N. Kami-ike
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Y. Takeuchi
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kishimoto, U., Kami-ike, N. & Takeuchi, Y. The role of electrogenic pump inChara corallina . J. Membrain Biol. 55, 149–156 (1980). https://doi.org/10.1007/BF01871157

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01871157

Keywords

Search

Navigation