The Journal of Membrane Biology

, Volume 107, Issue 1, pp 57–62 | Cite as

Na+(Li+)/Branched-chain amino acid cotransport inPseudomonas aeruginosa

  • Yoshihiko Uratani
  • Tomofusa Tsuchiya
  • Yukiko Akamatsu
  • Toshimitsu Hoshino
Articles

Summary

A transport system for branched-chain amino acids (designated as LIV-II system) inPseudomonas aeruginosa requires Na+ for its operation. Coupling cation for this system was identified by measuring cation movement during substrate entry using cation-selective electrodes. Uptakes of Na+ and Li were induced by the imposition of an inwardly-directed concentration gradient of leucine, isoleucine, or valine. No uptake of H was found, however, under the same conditions. In addition, effects of Na+ and Li+ on the kinetic property of the system were examined. At chloride salt concentration of 2.5mm, values of apparentKm andVmax for leucine uptake were larger in the presence of Na+ than Li+. These results indicate that the LIV-II transport system is a Na+(Li+)/substrate cotransport system, although effects of Na+ and Li+ on kinetics of the system are different.

Key Words

Pseudomonas aeruginosa cation cotransport branched-cham amino acids 

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Copyright information

© Springer-Verlag New York Inc. 1989

Authors and Affiliations

  • Yoshihiko Uratani
    • 1
  • Tomofusa Tsuchiya
    • 2
  • Yukiko Akamatsu
    • 2
  • Toshimitsu Hoshino
    • 1
  1. 1.Mitsubishi Kasei Institute of Life SciencesMachida-shi, TokyoJapan
  2. 2.Department of Microbiology, Faculty of Pharmaceutical SciencesOkayama UniversityOkayamaJapan

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