Summary
During progesterone-stimulated maturation of defolliculated full-grownXenopus oocytes, the activities of the transport systems forl-alanine, thymidine, chloride, phosphate, and alkali ions decrease. Differences of the extent and time course of these changes suggest that they are controlled by at least partially independent mechanisms.
A closer investigation of the Na-K ATPase has shown that in unstimulated oocytes, ouabain produces maximal inhibition when 8–12×109 molecules are bound per cell. This number is bound during the first phase of a diphasic uptake process. Since this phase can be suppressed by increasing the concentration of external K+ to 45 mmol/liter or more, it is concluded that it refers to binding to the Na−K pump in the plasma membrane. Ouabain bound prior to progesterone-induced germinal vesicle breakdown (GVBD) remains bound after the breakdown, although the Na−K pump loses the capacity to bind ouabain after GVBD in oocytes that had not been exposed to ouabain preceding GVBD. In the presence of Mg++ membranes isolated before regulatory inhibition of pumping and ouabain binding show a Na+-dependent incorporation of32P from γ-[32P]-ATP that can be reversed by the addition of K+. The phosphorylation site migrates on LiDS-polyacrylamide gel electropherograms at about 98,000 daltons and can be identified as a Commassie blue-stainable band.
Similar content being viewed by others
References
Bellé, R., Marot, J., Ozon, R. 1976. Nature of progesterone action on amino acid uptake by isolated full-grown oocytes ofXenopus laevis.Biochim. Biophys. Acta 419:342–348
Bellé, R., Ozon, R., Stinnakre, J. 1977. Free calcium in full-grownXenopus laevis oocytes following treatment with ionophore A23187 or progesterone.Mol. Cell. Endocrinol. 8:65–72
Benos, D.J. 1981. Ouabain binding to preimplantation rabbit blastocysts.Dev. Biol. 83:69–78
Bodemann, H.H., Hoffman, J.F. 1976. Comparison of the side-dependent effects of Na and K on orthophosphate-, UTP-and ATP-prometed ouabain binding to reconstituted red blood cell ghosts.J. Gen. Physiol. 67:527–545
Carvallo, P., Albuja, C.M. de, Allende, C.C., Allende, J.E. 1981. Hormonal regulation of glucose uptake by amphibian follicles.Exp. Cell Res. 136:215–223
Cook, J.S., Tate, E.H., Shaffer, C. 1982. Uptake of3H-ouabain from the cell surface into the lysosomal compartment of Hela cells.J. Cell. Physiol. 110:84–92
Dixon, J.F., Hokin, L.E. 1974. Studies on the characterization of the sodium-potassium transport adenosine triphosphatase. Purfication and properties of the enzyme from the electric organ ofElectrophorus electricus.Arch. Biochem. Biophys. 163:749–758
Ecker, R.E., Smith, L.D. 1971. Influence of exogenous ions on the events of maturation inRana pipiens oocytes.J. Cell. Physiol. 77:61–70
Jung, D., Richter, H.-P. 1983. Changes of alanine-sodium cotransport during maturation ofXenopus laevis oocytes.Cell Biol. I.R. 7:697–707
Kado, R.T., Marcher, K., Ozon, R. 1981. Electrical membrane properties of theXenopus laevis oocyte during progesterone-induced meiotic maturation.Dev. Biol. 84:471–476
Knauf, P.A., Proverbio, F., Hoffman, J.F. 1974. Chemical characterization and pronase susceptibility of the Na∶K pump-associated phosphoprotein of human red blood cells.J. Gen. Physiol. 63:305–323
Morrill, G.A., Ziegler, D. 1980. Na+ and K+ uptake and exchange by the amphibian oocyte during the first meiotic division.Dev. Biol. 74:216–223
Pennequin, P., Schorderet-Slatkine, S., Drury, K.C., Baulieu, E.E. 1975. Decreased uptake of3H-leucine during progester-one-induced maturation ofXenopus laevis oocytes.FEBS Lett. 51:156–160
Prindle, K.H., Skelton, C.L., Epstein, S.E., Marcus, F.I. 1971. Influence of extracellular potassium concentration on myocardial and isotropic effect of tritiated digoxin.Circ. Res. 28:337–345
Richter, H.-P. 1980. SDS-polyacrylamide gel electrophoresis of isolated cortices ofXenopus laevis eggs.Cell Biol. I.R. 4:985–995
Richter, H.-P., Tintschl, A. 1983. Cortex and plasma membrane proteins ofXenopus oocytes.Cell Biol. I.R. 7:1105–1114
Smith, L.D. 1981. Frog oocytes and sea urchin eggs: Steroid hormones and sperm induce similar events.In: Progress in Developmental Biology. W. Sauer, editor. pp. 35–47 Gustav Fischer, Stuttgart-New York
Weinstein, S.P., Kostellow, A.B., Ziegler, D.H., Morrill, G.A. 1982. Progesterone-induced down-regulation of an electrogenic Na+, K+-ATPase during the first meiotic division in amphibian oocytes.J. Membrane Biol. 69:41–48
Ziegler, D., Morrill, G.A. 1977. Regulation of the amphibian oocyte plasma membrane ion permeability by cytoplasmatic factors during the first meiotic division.Dev. Biol. 60:318–325
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Richter, HP., Jung, D. & Passow, H. Regulatory changes of membrane transport and ouabain binding during progesterone-induced maturation ofXenopus oocytes. J. Membrain Biol. 79, 203–210 (1984). https://doi.org/10.1007/BF01871059
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01871059