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The Journal of Membrane Biology

, Volume 58, Issue 1, pp 43–55 | Cite as

Interaction of phlorizin and sodium with the renal brush-border membraned-glucose transporter: Stoichiometry and order of binding

  • R. James Turner
  • M. Silverman
Articles

Summary

The order and stoichiometry of the binding of phlorizin and sodium to the renal brush-border membraned-glucose transporter are studied. The experimental results are consistent with a random-binding sites is one-to-one. When the kinetics of phlorizin binding are measured as a function of increasing sodium concentration no significant variation is found in the apparent number of binding sites; however, the apparent binding constant for phlorizin decreases rapidly from approximately 16 μm at [Na]=0 to 0.1 μm at [Na]=100mm and approaches 0.05 μm as [Na]→∞. The experimental data are fit to a random carrier-type model of the coupled transport of sodium andd-glucose. A complete parameterization of the phlorizin binding properties of this model under sodium equilibrium conditions is given.

Keywords

Sodium Experimental Data Equilibrium Condition Significant Variation Human Physiology 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc. 1981

Authors and Affiliations

  • R. James Turner
    • 1
    • 2
  • M. Silverman
    • 1
    • 2
  1. 1.Laboratory of Kidney and Electrolyte MetabolismNational Heart, Lung, and Blood InstituteBethesda
  2. 2.Department of MedicineUniversity of TorontoTorontoCanada

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