The Journal of Membrane Biology

, Volume 58, Issue 1, pp 43–55 | Cite as

Interaction of phlorizin and sodium with the renal brush-border membraned-glucose transporter: Stoichiometry and order of binding

  • R. James Turner
  • M. Silverman


The order and stoichiometry of the binding of phlorizin and sodium to the renal brush-border membraned-glucose transporter are studied. The experimental results are consistent with a random-binding sites is one-to-one. When the kinetics of phlorizin binding are measured as a function of increasing sodium concentration no significant variation is found in the apparent number of binding sites; however, the apparent binding constant for phlorizin decreases rapidly from approximately 16 μm at [Na]=0 to 0.1 μm at [Na]=100mm and approaches 0.05 μm as [Na]→∞. The experimental data are fit to a random carrier-type model of the coupled transport of sodium andd-glucose. A complete parameterization of the phlorizin binding properties of this model under sodium equilibrium conditions is given.


Sodium Experimental Data Equilibrium Condition Significant Variation Human Physiology 
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Copyright information

© Springer-Verlag New York Inc. 1981

Authors and Affiliations

  • R. James Turner
    • 1
    • 2
  • M. Silverman
    • 1
    • 2
  1. 1.Laboratory of Kidney and Electrolyte MetabolismNational Heart, Lung, and Blood InstituteBethesda
  2. 2.Department of MedicineUniversity of TorontoTorontoCanada

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