The Journal of Membrane Biology

, Volume 63, Issue 3, pp 243–254 | Cite as

Noise analysis of the K+ current through the apical membrane ofNecturus gallbladder

  • Heinz Gögelein
  • Willy Van Driessche
Articles

Summary

Current noise power spectra of the voltage-clamped(V=0) Necturus gallbladder, exposed to NaCl-Ringer's on both sides contained a relaxation noise component, which overlapped with a 1/fα noise component, with α being about 2. Substitution of all Na+ by K+ on either the serosal or mucosal side increased the relaxation as well as the 1/fα noise component considerably. InNecturus gallbladder both noise components are reduced by addition of 10mm, 2,4,6-triaminopyrimidine (TAP) or 5mm Ba2+ to the mucosal side, as well as by acidification of the mucosal solution to pH5 and lower. Fivemm of tetraethylammonium (TEA+) added to the mucosal solution, abolished K+ relaxation noise and decreased the 1/fα noise component. Applying a Cs+ concentration gradient across the epithelium did not yield relaxation noise. However, if Rb+ was substituted for all Na+ on one side, a Lorentzian noise component appeared in the spectrum. Its plateau was smaller than with KCl-Ringer's on the respective side. These data confirm the existence of fluctuating K+ channels in the apical membrane of theNecturus gallbladder. Furthermore it can be concluded that these channels have the permeability sequence K+>Rb+>Cs+. The inhibition of the fluctuation by mucosal acidification indicates the existence of acidic sites in the channel. The singlechannel conductance was estimated to be between 6.5 and 40 pS.

Key words

gallbladder noise analysis potassium channel apical membrane Necturus 

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Copyright information

© Springer-Verlag New York Inc 1981

Authors and Affiliations

  • Heinz Gögelein
    • 1
  • Willy Van Driessche
    • 1
  1. 1.Laboratorium voor FysiologieKULLeuvenBelgium

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