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The Journal of Membrane Biology

, Volume 58, Issue 2, pp 155–160 | Cite as

Computer assisted analysis of ferritin-insulin receptor sites on adipocytes and the effects of cytochalasin B on groups of insulin receptor sites

  • Nahum D. Gershon
  • Robert M. Smith
  • Leonard Jarett
Articles

Summary

A computerized quantitative technique was used to analyze the distribution of ferritininsulin receptor sites on rat adipocytes and the effects of cytochalasin B on groups of receptor sites. Computer analysis of separation distances between receptor sites established that insulin receptor sites on adipocytes did not have a random distribution but have a distinct tendency to exist in groups with a maximum separation distance between particles of 400 Å. A peak in the distribution of separation distances occurred at 100–200 Å. Cytochalasin B, but not cytochalasin D, treatment of adipocytes resulted in a decrease in the number of large groups of receptor sites and a corresponding increase in single and paired receptor sites without affecting the separation distance between the remaining grouped receptors. This suggested that when cytochalasin B disrupted the bond holding receptor sites together, it caused complete disruption. These observations provided additional information on the ultrastructural characteristics of the insulin receptor. Further application of these techniques to the analysis of insulin receptors may provide the necessary structural correlates to the biochemically observed differences in insulin action in other tissues and diseased states.

Keywords

Insulin Receptor Separation Distance Insulin Action Cytochalasin Receptor Site 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc 1981

Authors and Affiliations

  • Nahum D. Gershon
    • 1
    • 2
  • Robert M. Smith
    • 1
    • 2
  • Leonard Jarett
    • 1
    • 2
  1. 1.Division of Computer Research and TechnologyNational Institutes of HealthBethesda
  2. 2.Division of Laboratory Medicine, Departments of Pathology and MedicineWashington University School of MedicineSt. Louis

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