Summary
Previous studies have shown that urea and acetamide traverse the erythrocyte membrane by way of facilitated diffusion. The nature of this selective pathway is unknown. The present studies investigate the effects of proteolytic enzymes and crosslinking agents on amide transport. Cleavage of the erythrocyte membrane surface by pronase or trypsin had no effect on urea and acetamide permeability or inhibition by phloretin. These findings suggest that the sialoglycopeptide segment of the sialoglycoproteins is not critical to urea and acetamide transport. In addition, extensive crosslinking of membrane proteins with glutaraldehyde had no effect on amide transport in the absence or presence of phloretin.
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Bender, W. W., Garan, H., Berg, H. C. 1971. Proteins of the human erythrocyte membrane as modified by pronase.J. Mol. Biol. 58: 783
Blumenfeld, O. O., Gallop, P. M., Liao, T. H. 1972. Modification and introduction of a specific radioactive label into the erythrocyte membrane sialoglycoprotein.Biochem. Biophys. Res. Commun. 48: 242
Cabantchik, Z. I., Rothstein, A. 1974. Membrane proteins related to anion permeability of human red blood cells.J. Membrane Biol. 15: 227
Capaldi, R. A. 1973. A crosslinking study of the beef erythrocyte membrane: Extensive interaction of all the proteins of the membrane except for the glycoproteins.Biochem. Biophys. Res. Commun. 50: 656
Dodge, J. T., Mitchell, C., Hanahan, D. J. 1963. The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes.Arch. Biochem. Biophys. 100: 119
Eggena, P. 1973. Inhibition of vasopressin stimulated urea transport across the toad bladder by thiourea.J. Clin. Invest. 52: 2963
Fairbanks, G., Steck, T. L., Wallach, B. F. H. 1971. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.Biochemistry 10: 2606
Herz, F. 1968. On the effects of tannic acid on erythrocyte membrane acetylcholinesterase.Proc. Soc. Exp. Biol., N. Y. 127: 1240
Herz, F., Kaplan, E. 1968. Effects of tannic acid on erythrocyte enzymes.Nature 217: 1258
Hunter, F. R., George, J., Ospina, B.. 1965. Possible carriers in erythrocytes.J. Cell. Comp. Physiol. 65: 299
Kaplan, M. A., Hays, L., Hays, R. M. 1974. The evolution of a facilitated diffusion pathway for amides in the erythrocyte.Amer. J. Physiol. 226: 1327
Levine, S., Franki, N., Hays, R. M. 1973. Effects of phloretin on water and solute movement in the toad bladder.J. Clin. Invest. 52: 1435
Macey, R. I., Farmer, R. E. L. 1970. Inhibition of water and solute permeability in human red cells.Biochim. Biophys. Acta 211: 104
Rabinowitz, L., Gunther, R. A. 1973. Urea transport in elasmobranch erythrocytes.Amer. J. Physiol. 224: 1109
Shuchter, S. H., Franki, N., Hays, R. M. 1973. The effect of tanning agents on the permeability of the toad bladder to water and solutes.J. Membrane Biol. 14: 177
Steck, T. L. 1972. Crosslinking the major proteins of the isolated erythrocyte membrane.J. Mol. Biol. 66: 295
Tishler, P. V., Epstein, C. J. 1968. A convenient method of preparing polyacrylamide gels for liquid scintillation spectrometry.Analyt. Biochem. 22: 89
Triplett, R. B., Carraway, K. L. 1972. Proteolytic digestion of erythrocytes, resealed ghosts, and isolated membranes.Biochemistry 11: 2897
Whittam, R. 1964. The diffusion and facilitated transfer of non-electrolytes.In: Transport and Diffusion in R.B.C. Williams and Wilkin Company, Baltimore
Wieth, J. O., Funder, J., Gunn, R. B., Brahm, J. 1974. Passive transport pathways for chloride and urea through the red cell membrane.In: Comparative Biochemistry and Physiology of Transport. L. Balix, editor. North Holland Publishing Company, Amsterdam
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Kaplan, M.A., Hays, R.M. & Blumenfeld, O.O. Membrane proteins and urea and acetamide transport in the human erythrocyte. J. Membrain Biol. 20, 181–190 (1975). https://doi.org/10.1007/BF01870635
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DOI: https://doi.org/10.1007/BF01870635