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The mechanism of Cl− transport at the plasma membrane ofChara corallina I. Cotransport with H+

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Summary

Cl− transport into cells ofChara corallina was studied in relation to that of other ions which have been proposed as cosubstrates for the Cl− transport system. Although there appears to be a partial mutual dependence between K+ and Cl− for transport in intact cells, this is not apparent in cells which have been perfused internally. Moreover, in intact cells, the fluxes of K+ and Cl− show a large degree of independence in their responses to Cl− starvation. Cl− transport is electrogenic in a direction indicating the transport of excess positive charge into the cell. In the absence of any other likely counter ion, it is suggested that Cl− is cotransported with H+. Response of Cl− influx to internal and external pH in perfused cells is consistent with this suggestion. There appears, in addition, to be a role for ATP in transport as judged by fourfold stimulation of Cl− influx in perfused cells when 1mm ATP is incorporated in the perfusion medium.

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  1. Dale Sanders

    Present address: Department of Physiology, Yale Medical School, 333 Cedar Street, 06510, New Haven, Connecticut

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  1. Botany School, Downing Street, CB2 3EA, Cambridge, UK

    Dale Sanders

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Sanders, D. The mechanism of Cl− transport at the plasma membrane ofChara corallina I. Cotransport with H+ . J. Membrain Biol. 53, 129–141 (1980). https://doi.org/10.1007/BF01870581

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  • DOI: https://doi.org/10.1007/BF01870581

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