The Journal of Membrane Biology

, Volume 70, Issue 3, pp 239–251 | Cite as

Hormonal regulation of cell junction permeability: Upregulation by catecholamine and prostaglandin E1

  • A. Radu
  • G. Dahl
  • W. R. Loewenstein
Articles

Summary

By cellular activation with hormones, we test the proposition (Loewenstein, W.R.,Physiol. Rev.61:829, 1981) that the permeability of cell junction is upregulated through elevation of the level of cyclic AMP. Cultured rat glioma C-6 cells, with β-adrenergic receptors, and human lung WI-38 cells, with prostaglandin receptors, were exposed to catecholamine (isoproterenol) and prostaglandin E1, respectively, while their junctions were probed with microinjected fluorescent-labelled mono-, di-, and triglutamate. Junctional permeability, as indexed by the proportion of cell interfaces transferring the probes, rose after the hormone treatments. The increase in permeability took several hours to develop and was associated with an increase in the number of gap-junctional membrane particles (freeze-fracture electron microscopy). Such interaction between hormonal and junctional intercellular communication may provide a mechanism for physiological regulation of junctional communication and (perhaps as part of that) for physiological coordination of responses of cells in organs and tissues to hormones.

Key Words

cell junction permeability cell-to-cell communication membrane permeability cell-to-cell membrane channels gap junction hormones catecholamine prostaglandin E1 cyclic AMP 

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Copyright information

© Springer-Verlag New York Inc 1982

Authors and Affiliations

  • A. Radu
    • 1
  • G. Dahl
    • 1
  • W. R. Loewenstein
    • 1
  1. 1.Department of Physiology and BiophysicsUniversity of Miami School of MedicineMiami

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