The Journal of Membrane Biology

, Volume 54, Issue 3, pp 173–181 | Cite as

Characterization of the lipid and polypeptide components of a tetrodotoxin binding membrane fraction fromElectrophorus electricus

  • Mary-Ann Kallai-Sanfacon
  • Juta K. Reed
Article

Summary

This paper reports an analysis of the lipid and polypeptide composition of a tetrodotoxin (TTX)-binding plasma membrane fraction of the eel electroplaque. Phospholipids comprise 73% of the total lipid with cholesterol and neutral glycerides constituting about 21 and 6%, respectively. The major phospholipids are phosphatidylcholine (47.3%), phosphatidylethanolamine (32.6%), phosphatidylserine (13.1%), and sphingomyelin (4.5%). Phosphatidylinositol and phosphatidic acid are minor components. Plasmalogens comprise approximately 19% of the total phosphatidylethanolamine. Each major phospholipid class was analyzed for fatty acyl composition. The results indicate a unique distribution profile for each class with respect to chain length and unsaturation. PE and PS both contain high percentages of polyunsaturated fatty acids particularly docosahexaenoic acid with constitutes 35 and 39% of the total fatty acids, respectively. However, PC and PS contain significantly lower levels of polyunsaturated fatty acids. The lipid profile observed in this preparation is compared to those previously reported for membranes from other excitable tissues. Polyacrylamide gel electrophoresis of the membranes indicates a complex distribution of peptides with several major species and at least 30 minor components. Two of the major species have molecular weights corresponding to those of the two subunits of the (Na++K+)-ATPase.

Keywords

Glyceride Membrane Fraction Phosphatidylethanolamine Docosahexaenoic Acid Phosphatidic Acid 

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Copyright information

© Springer-Verlag New York Inc 1980

Authors and Affiliations

  • Mary-Ann Kallai-Sanfacon
    • 1
  • Juta K. Reed
    • 1
  1. 1.Department of Chemistry, Erindale CollegeUniversity of TorontoTorontoCanada

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