Summary
The temperature and pH dependence of water exchange has been studied on isolated erythrocytes suspended in isotonic buffered solutions. At pH 7.4 a break in the Arrhenius plot of water exchange time at around 26°C was found. The mean value of the apparent activation energy of the water exchange time at temperatures higher than that of the discontinuity was 5.7 kcal/mole (±0.4); at lower temperatures the values of the apparent activation energy were below 1.4 kcal/mole. The pH dependence of water exchange time of isolated erythrocytes revealed a marked increase of the water exchange time values in the acid range of pH; a much smaller variation of the same parameter occurs between pH 7.0 and 8.0. These finding could be correlated with other processes involving erythrocyte membranes that showed similar pH and temperature dependence and were considered to indicate state transitions in the membranes. It is suggested that the temperature and pH effects on water diffusion indicate that conformational changes and cooperative effects are implicated in the mechanism of this transport process.
Similar content being viewed by others
References
Aloni, B., Eitan, A., Livne, A. 1977 The erythrocyte membrane site for the effect of temperature on osmotic fragility.Biochim. Biophys. Acta 465:46
Benga, G., Strach, S.J. 1975. Interpretation of the electron spin resonance spectra of nitroxide-maleimide-labeled proteins and the use of this technique in the study of albumin and biomembranes.Biochim. Biophys. Acta 400:69
Bieri, V.G., Wallach, D.F.H. 1976. Lipid-protein relationships in erythrocyte membranes revealed by paramagnetic quenching of protein fluorescence.Biochim. Biophys. Acta 443:198
Bond, J.D., Baumann, G. 1979. Comments on electrostriction and/or cooperativity as viable models of membrane excitability.Physiol. Chem. Phys. 10:381
Brahm, J. 1977. Temperature-dependent changes of chloride transport kinetics in human red cells.J. Gen. Physiol. 70:283
Brahm, J., Wieth, J.O. 1977. Separate pathways for urea and water, and for chloride in chicken erythrocytes.J. Physiol. (London) 266:727
Brown, P.A., Feinstein, M.B., Sha'afi, R.I. 1975. Membrane proteins related to water transport in human erythrocytes.Nature (London) 254:523
Buckley, J.T., Hawthorne, J.N. 1972. Erythrocyte membrane polyphosphoinositide metabolism and the regulation of calcium binding.J. Biol. Chem. 247:7218
Conlon, T., Outhred, R. 1972. Water diffusion permeability of erythrocytes using an NMR technique.Biochim. Biophys. Acta 288:354
Conlon, T., Outhred, R. 1978. The temperature dependence of erythrocyte water diffusion permeability.Biochim. Biophys. Acta 511:408
Davis, J.H., Maraviglia, B., Weeks, G., Godin, D.V. 1979. Bilayer rigidity of the erythrocyte membrane2H — NMR of a perdeuterated palmitic acid probe.Biochim. Biophys. Acta 550:362
De Gier, J. 1979. Membrane lipids and the general permeability properties of the interface.In: Advances in Inflamation Research. G. Weissmann, editor. p. 15. Raven Press, New York
Fairbanks, G., Steck, T.L., Wallach, D.F.H. 1971. Electrophoretic analysis of the major polipeptides of the human erythrocyte membrane.Biochemistry 10:2606
Feinstein, M.B., Fernandez, S.M., Sha'afi, R.I. 1975. Fluidity of natural membranes and phosphatidylserine and ganglioside dispersions. Effects of local anaesthetics, cholesterol and protein.Biochim. Biophys. Acta 413:354
Goekoop, J.G., Spies, F., Wisse, D.M., Vrielink, R., De Vries, E., Van Kempen, G.M.J. 1978. pH-dependent behaviour of erythrocyte membrane elevations., II. Occurrence in moderate acidemic and alkalenic conditions.Cell Biol. Int. Rep. 2:397
Inesi, G., Millman, M., Eletr, S. 1973. Temperature-induced transitions of function and structure in sarcoplasmic reticulum membranes.J. Mol. Biol. 81:483
Jennings, M.L. 1978. Characteristics of CO2-independent pH equilibration in human red blood cells.J. Membrane Biol. 40:365
Jennings, M.L., Passow, H. 1979. Anion transport accross the erythrocyte membrane,in situ proteolysis of band 3 protein, and cross-linking of proteolytic fragments by 4,4′-diisothiocyano-dihydrostilbene-2,2′-disulfonate.Biochim. Biophys. Acta 554:498
Johnson, R.M. 1975. The kinetics of resealing of washed erythrocyte ghosts.J. Membrane Biol. 22:231
Lacko, L., Witke, B., Geck, P. 1973. The temperature dependence of the exchange transport of glucose in human erythrocytes.J. Cell. Physiol. 82:213
Lenaz, G. 1973. The role of lipids in the regulation of membrane-associated activities.Acta Vitaminol. Enzymol. 27:62
Lenaz, G., Curatola, G., Massotti, L. 1975. Perturbation of membrane fluidity.J. Bioenerg. 7:223
Macey, R.I., Farmer, R.E.L. 1970. Inhibition of water and solute permeability in human red cells.Biochim. Biophys. Acta 211:104
Marchesi, V.T., Tillack, T.W., Jackson, R.L. 1972. Chemical characterisation and surface orientation of the major glycoproteins of the human erythrocyte membrane.Proc. Nat. Acad. Sci. USA 69:1445
Morariu, V.V., Benga, G. 1977. Evaluation of a nuclear magnetic resonance technique for the study of water exchange through erythrocyte membranes in normal and pathological subjects.Biochim. Biophys. Acta 469:301
Raison, J.K., Lyons, J.M., Thomson, W.W. 1971. The influence of membranes on the temperature-induced changes in the kinetics of some regulatory enzymes of mitochondria.Arch. Biochem. Biophys. 142:83
Sha'afi, R.I. 1977, Water and small nonelectrolyte permeation in red cells.In: Membrane Transport in Red Cells. C. Ellory, editor. p. 221. Academic Press, New York
Shporer, M., Civan, M.M. 1975. NMR study of17O from H2 17O in human erythrocyte.Biochim. Biophys. Acta 385:81
Traüble, H 1972. Biomembranes. Phase transitions in lipidsIn: Passive Permeability of Cell Membranes. F. Kreuzer and J.F.G. Sledgers, editors. Vol. 3, p. 197. Plenum Press, New York-London
Van Deenen, L.L.M., De Gier, J., Demel, R.A., De Kruiff, B., Blok, M.C., Van der Neut-Kok, E.C.M., Haest, C.W.M., Ververgaert, P.H.J.Th., Verleij, A.J. 1975. Lipid-lipid and lipid-protein interaction in model systems and membranes.Ann. N. Y. Acad. Sci. 264:124
Verma, S.P., Wallach, D.F.H. 1976. Erythrocyte membranes undergo cooperative, pH-sensitive state transitions in the physiological temperature range: Evidence from Raman spectroscopy.Proc. Nat. Acad. Sci. USA 73:3558
Vieira, F.L., Sha'afi, R., Solomon, A.K. 1970. The state of water in human and dog red blood cell membranes.J. Gen. Physiol. 55:451
Volkenstein, M.V. 1977. Molecular Biology, p. 33. Academic Press, New York
Zimmer, G., Schirmer, H. 1974. Viscosity changes of erythrocyte membrane and membrane lipids at transition temperature.Biochim. Biophys. Acta 345:314
Author information
Authors and Affiliations
Additional information
Institute for Isotopic and Molecular Technology.
Rights and permissions
About this article
Cite this article
Morariu, V.V., Pop, V.I., Popescu, O. et al. Effects of temperature and pH on the water exchange through erythrocyte membranes: Nuclear magnetic resonance studies. J. Membrain Biol. 62, 1–5 (1981). https://doi.org/10.1007/BF01870194
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01870194