The Journal of Membrane Biology

, Volume 12, Issue 1, pp 145–158 | Cite as

ATP-driven chloride pumping and ATPase activity in theLimonium salt gland

  • B. S. Hill
  • A. E. Hill


Using the short-circuit current as a measure of the electrogenic chloride transport in the salt glands ofLimonium, the effects of various inhibitors, of light-dark changes and of oxygen removal have been studied during steady-state pumping. The results are consistent with the hypothesis that ATP is the energy source for the chloride pump in this system.

When microsomes from salt-loaded tissue are tested for ATPase activity, a substantial fraction of this is found to be chloride-stimulated. In uninduced tissue the Cl-ATPase activity is very much lower, and the induction by salt-loading can be blocked by puromycin. The parallels with Cl-pumping in this tissue are close enough to assume that the Cl-ATPase activity is that of the pump itself; the way is therefore open to study the pumpin vitro.


Oxygen Chloride Energy Source Human Physiology ATPase Activity 
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Copyright information

© Springer-Verlag New York Inc 1973

Authors and Affiliations

  • B. S. Hill
    • 1
    • 2
  • A. E. Hill
    • 1
    • 2
  1. 1.Life Science InstituteThe Hebrew UniversityJerusalem
  2. 2.The Botany SchoolUniversity of CambridgeCambridgeEngland

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