Recovery of galactosyltransferase activity from sucrose gradients during isolation of Golgi membranes
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The purification of Golgi membranes has been characterized in membrane fractions isolated from rat liver by homogenization and by differential centrifugation on a discontinuous sucrose gradient. The purification of membranes, judged from the ratio of galactosyltransferase in the Golgi-rich fractions to that in the liver homogenate, correlated with the proportion of structurally intact isolated membranes. With increasing purification of membrane fractions, there was a corresponding increase in the total recovery of enzyme activity. It is postulated that this may reflect not only an increase in the purification of membranes but may involve the presence of specific galactosyltransferase inhibitors in intracellular membranes of the liver.
KeywordsSucrose Enzyme Activity Human Physiology Membrane Fraction Liver Homogenate
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