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Pulse NMR studies of water permeability in phosphatidylcholine vesicles containing general anesthetics

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Summary

Phosphatidylcholine was extracted from egg yolks and sonicated withn-alkyl alcohols to give vesicles. The magnetic environment of the external compartment was then modified by the addition of shift reagent (Co2+). This allowed determination of the average lifetime for exchange of water molecule protons by observing the relaxation rate dependence on the interval between pulses in a Carr-Purcell sequence. For vesicles containingn-hexanol as 33% of lipid, the average water molecule lifetime in the internal compartment was found to be substantially less than that for a vesicle without hexanol. The lifetime, 11.3±4 msec, is equivalent to a permeability coefficient of 60 μ/sec at 34°C.

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References

  1. Allerhand, A., Gutowsky, H.S. 1964. Spin echo NMR studies of chemical exchange.J. Chem. Phys. 41:2115

    Google Scholar 

  2. Andrasko, J., Forsen, S. 1974. NMR study of rapid water diffusion across lipid bilayers in dipalmitoyl lecithin vesicles.Biochem. Biophys. Res. Commun. 60:813

    Google Scholar 

  3. Bangham, A.D., DeGier, J., Greville, G.O. 1967. Osmotic properties and water permeability of phospholipid crystals.Chem. Phys. Lipids 1:225

    Google Scholar 

  4. Bangham, A.D., Hill, M.W., Miller, N. 1974. Preparation and use of Liposomes as models of biological membranes.Methods Membr. Biol. 1:1

    Google Scholar 

  5. Bangham, A.D., Standish, N.M., Miller, N. 1965. Cation permeability of phospholipid membranes: Effect of narcotics.Nature (London) 208:1295

    Google Scholar 

  6. Cass, A., Finkelstein, A. 1967. Water permeability of thin lipid membranes.J. Gen. Physiol 50:1765

    Google Scholar 

  7. Farrar, T.C., Becker, E.D. 1971. Pulse and Fourier Transform NMR. Academic Press, New York

    Google Scholar 

  8. Gent, M.P.N., Prestegard, J.H. 1974. Cholesterol-phosphatidylcholine interactions in vesicle systems.Biochemistry 13:4027

    Google Scholar 

  9. Huang, C. 1969. Studies on phosphatidylcholine vesicles. Formation and physical characteristics.Biochemistry 8:344

    Google Scholar 

  10. Levine, Y.K. 1972. Physical studies of membrane structure.Prog. Biophys. Mol. Biol. 24:3

    Google Scholar 

  11. Papahadjapoulos, D., Kimelberg, H.K. 1973. Phospholipid vesicles as models for biological membranes.Prog. Surf. Sci. 4(II):141

    Google Scholar 

  12. Reeves, J.P., Dowben, R.M. 1970. Water permeability of phospholipid vesicles.J. Membrane Biol. 3:123

    Google Scholar 

  13. Singleton, W.S., Gray, M.S., Brown, M.L., White, J.L. 1965. Chromatographically homogeneous lecithin from egg phospholipids.J. Am. Oil. Chem. Soc. 42:53

    Google Scholar 

  14. Thompson, T.E., Henn, F.A. 1970. Experimental phospholipid model membranes.In: Membranes of Mitochondria and Chloroplasts. E. Racker, editor. pp. 1–52. Van Nostrand and Reinhold, New York

    Google Scholar 

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Authors and Affiliations

  1. Department of Chemistry, Yale University, Sterling Chemistry Laboratory, 06520, New Haven, CT

    Howard S. Hochster & J. H. Prestegard

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  1. Howard S. Hochster
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  2. J. H. Prestegard
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Hochster, H.S., Prestegard, J.H. Pulse NMR studies of water permeability in phosphatidylcholine vesicles containing general anesthetics. J. Membrain Biol. 35, 303–307 (1977). https://doi.org/10.1007/BF01869955

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  • DOI: https://doi.org/10.1007/BF01869955

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