The Journal of Membrane Biology

, Volume 35, Issue 1, pp 189–204 | Cite as

Redox involvement in acid secretion in the amphibian gastric mucosa

  • Edd C. Rabon
  • H. M. Sarau
  • W. S. Rehm
  • G. Sachs


Gastric fundic metabolism was studied by spectroscopic observation in frog mucosa during transitions of secretory status in vitro and by direct measurement of pyridine nucleotides and associated metabolites in biopsies of dog fundic mucosa also during secretory oxidation of the redox components from flavin adenine dinucleotide (FAD) to cytochromea3. Addition of histamine resulted in reduction of these components with onset of secretion by about 50%. In contrast, the effect of apparently, burimamide and subsequently histamine on the ratio of nicotinamide adenine dinucleotide to nicotinamide adenine dinucleotide, reduced (NAD+/NADH) was relatively slight. Further, the presence of burimamide substantially reduces the effect of amytal on the pyridine nucleotide spectrum and abolishes the effect of amytal on FAD and the cytochromes. Measurements of lactate, pyruvate, α-ketoglutarate, NH3 and glutamate in the dog showed that whereas the calculated NAD+/NADH ratio in the cytoplasm declined with onset of secretion, the calculated mitochondrial ratio rose. No change was noted in the nicotinamide adenine dinucleotide phosphate/nicotinamide adenine dinucleotide phosphate, reduced (NADP+/NADPH) ratio. It is concluded that (1) H2 antagonists act by blocking substrate flow into the mitochondrial respiratory chain, (2) conversely, histamine stimulation acts at the level of substrate mobilization, and (3) there may be a cross-over in the mitochondrial chain between NAD+ and FAD.


Histamine NADH Dinucleotide NADP Nicotinamide Adenine Dinucleotide 
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  1. 1.
    Black, J.W., Duncan, W.A.M., Durant, C.J., Ganellin, C.R., Parsons, E.M. 1972. Definition and antagonism of histamine H2 receptors.Nature (London) 236:385Google Scholar
  2. 2.
    Chance, B., Williams, G.R. 1955. Respiratory enzymes in oxidative phosphorylation.J. Biol. Chem. 217: 395Google Scholar
  3. 3.
    Durbin, R.P., Michelangeli, F., Michel, A. 1974. Active transport and ATP in frog gastric mucosa.Biochim. Biophys. Acta 367:177Google Scholar
  4. 4.
    Folbegrava, J., Passoneau, J.V., Lowry, O.H., Schulz, D.W. 1969. Glycogen, ammonia and related metabolites in the brain during seizures evoked by methionine sulphoximine.J. Neurochem. 16:191Google Scholar
  5. 5.
    Ganser, A.L., Forte, J.G. 1973. K+-stimulated ATPase in purified microsomes of bullfrog oxyntic cells.Biochim. Biophys. Acta 307:169Google Scholar
  6. 6.
    Greenbaum, A.L., Gumaa, K.A., McLean, P. 1971. The distribution of hepatic metabolites and the control of the pathways of carbohydrate metabolism in animals of different dietary and hormonal status.Arch. Biochem. Biophys. 143:617Google Scholar
  7. 7.
    Hansford, R.G. 1972. Some properties of pyruvate and 2-oxoglutarate oxidation by blowfly flight-muscle mitochondria.Biochem. J. 172:271Google Scholar
  8. 8.
    Hersey, S.J. 1971. The energetic coupling of acid secretion in gastric mucosa.Phil. Trans. R. Soc. London B 262:261Google Scholar
  9. 9.
    Hersey, S.J. 1974. Interactions between oxidative metabolis and acid secreation in gastric mucosa.Biochim. Biophys. Acta 344:157Google Scholar
  10. 10.
    Hersey, S.J., High, W.L. 1971. On the mechanism of acid secretion inhibition by acetazolamide.Biochim. Biophys. Acta 233:604Google Scholar
  11. 11.
    Kidder, G.W., Curran, P.F., Rehm, W.S. 1966. Interactions between cytochrome system and H ion secretion in bullfrog gastric mucosa.Am. J. Physiol. 211:513Google Scholar
  12. 12.
    Loewenstein, J.M. 1969. Aspartate: Determination with glutamate-oxaloacetate transaminase and malate dehydrogenaseIn: Methods in Enzymology. J.M. Loewenstein, editor. Vol. XIII, p. 473. Academic Press Inc., New YorkGoogle Scholar
  13. 13.
    Lowry, O.H., Passoneau, J.V. 1972. Flexible Method of Enzymatic Analysis. Academic Press Inc., New YorkGoogle Scholar
  14. 14.
    Rehm, W.S. 1972. Proton transport.In: Metabolic Pathways. D.M. Greenberg, editor. Vol. 6, p. 187. Academic Press Inc., New YorkGoogle Scholar
  15. 15.
    Sachs, G., Chang, H.H., Rabon, E., Schackmann, R., Lewin, M., Saccomani, G. 1977. A non-electronic H+ pump in plasma membranes of hog stomach.J. Biol. Chem. (in press) Google Scholar
  16. 16.
    Sachs, G., Rabon, E., Saccomani, G., Sarau, H.M. 1972. Redox and ATP in acid secretion.Ann. N.Y. Acad. Sci. 246:456Google Scholar
  17. 17.
    Sachs, G., Wiebelhaus, V.D., Blum, A.L., Hirschowitz, B.I. 1972. Role of ATP and ATPase in gastric secretion.In: Gastric Secretion. G. Sachs, E. Heinz, and K.J. Ullrich, editors. p. 321. Academic Press, New YorkGoogle Scholar
  18. 18.
    Sarau, H.M., Foley, J., Moonsamy, G., Wiebelhaus, V.D., Sachs, G. 1975. Metabolism of dog gastric mucosa.J. Biol. Chem. 250:8321Google Scholar
  19. 19.
    Williamson, J.R., Ohkawa, K., Meijer, A.J. 1974. Regulation of ethanol oxidation in isolated rat liver cells.In: Alcohol and Aldehyde Metabolizing Systems. R.G. Thurman, T. Yonetani, J.R. Williamson, and B. Chance, editors. p. 365. Academic Press Inc., New YorkGoogle Scholar

Copyright information

© Springer-Verlag New York Inc. 1977

Authors and Affiliations

  • Edd C. Rabon
    • 1
    • 2
  • H. M. Sarau
    • 1
    • 2
  • W. S. Rehm
    • 1
    • 2
  • G. Sachs
    • 1
    • 2
  1. 1.Laboratory of Membrane BiologyUniversity of Alabama in BirminghamBirmingham
  2. 2.SKF LaboratoriesPhiladelphia

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