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Ca ionophore-stimulated ion secretion in rabbit ileal mucosa: Relation to actions of cyclic 3′,5′-AMP and carbamylcholine

The Journal of Membrane Biology volume 35, pages 159–173 (1977)Cite this article

Summary

Addition of the Ca ionophore, A23187 (0.5 μg/ml) to the serosal side of stripped rabbit ileal mucosa, produced changes in ion transport qualitatively identical with those produced by cyclic 3′,5′-AMP (cAMP) and theophylline: an increase in short-circuit current and resistance, net secretion of Cl due both to a decrease in the unidirectional mucosa (m) to serosa (s) flux and an increase in the (s) to (m) flux, and net secretion of Na due to a decrease in (m) to (s) flux. Measurements of intracellular cAMP level demonstrated no change following incubation with the ionophore. Removal of Ca from the serosal bathing medium diminished the effects of A23187 but did not impair the action of theophylline. Furthermore, removal of Ca from both the mucosal and serosal bathing media by replacing it with Sr completely abolished the p.d. response to A23187. These results suggest that the ionophore elicits its secretory actions by increasing Ca influx into the epithelial cells. In a similar way, carbamylcholine and serotonin, secretagogues known to have no effect on intracellular cAMP level in intestinal mucosa, were shown to be dependent on extracellular Ca to produce their full electrical response (although, in the case of carbamylcholine at least, Sr can substitute for Ca). In contrast, the secretagogues vasoactive intestinal peptide and prostaglandin E1, which raise cAMP concentration in intestinal mucosa, do not appear to require external Ca. It is interesting to speculate that Ca is an intracellular mediator of intestinal ion and water secretion and that some intestinal secretagogues may act as Ca ionophores.

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  1. Department of Medicine, Thorndike Laboratory and Gastrointestinal Unit of Beth Israel, Hospital and Harvard Medical School, 02215, Boston, Massachusetts

    Jennifer E. Bolton & Michael Field

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  1. Jennifer E. Bolton
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  2. Michael Field
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Bolton, J.E., Field, M. Ca ionophore-stimulated ion secretion in rabbit ileal mucosa: Relation to actions of cyclic 3′,5′-AMP and carbamylcholine. J. Membrain Biol. 35, 159–173 (1977). https://doi.org/10.1007/BF01869947

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  • DOI: https://doi.org/10.1007/BF01869947

Keywords

  • Theophylline
  • Intestinal Mucosa
  • Vasoactive Intestinal Peptide
  • A23187
  • Intracellular cAMP Level