The Journal of Membrane Biology

, Volume 28, Issue 1, pp 121–142 | Cite as

Effects of 2-deoxy-d-glucose, amiloride, vasopressin, and ouabain on active conductance andENa in the toad bladder

  • C. D. Hong
  • A. Essig


The effects of various agents on active sodium transport were studied in the toad bladder in terms of the equivalent circuit comprising an active conductanceKa, an electromotive forceENa, and a parallel passive conductanceKp. For agents which affectKa, but notENa orKp, the inverse slope of the plot of total conductance κ against short-circuit currentI0 evaluatesENa, and the intercept representsKp. Studies employing 5×10−7m amiloride to depressKa indicate a changingENa, invalidating the use of the slope technique with this agent. An alternative suitable technique employs 10−5m amiloride, which reducesI0 reversibly to near zero without effect onKp. Despite curvilinearity of the κ-I0 plot under these conditions,Kp may therefore be estimated fairly precisely from the residual conductance. It then becomes possible to follow the dynamic behavior ofKa andENa (in the absence of 10−5m amiloride) by frequent measurements of κ andI0, utilizing the relationshipsKa=K-Kp, andKNa=IO/(K-Kp). 2-deoxy-d-glucose (7.5×10−3m) depressedKa without affectingENa. Amiloride (5×10−7m) depressedKa and enhancedENa. Vasopressin (100 mU/ml) enhancedKa markedly and depressedENa slightly. Ouabain (10−4m) depressed bothKa andENa. All of the above effects were noted promptly;Kp was unaffected. The “electromotive force of Na transport”ENa appears not to be a pure energetic parameter, but to reflect kinetic factors as well, in accordance with thermodynamic considerations.


Active Sodium Vasopressin Equivalent Circuit Ouabain Frequent Measurement 
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Copyright information

© Springer-Verlag New York Inc. 1976

Authors and Affiliations

  • C. D. Hong
    • 1
  • A. Essig
    • 1
  1. 1.Department of PhysiologyBoston University School of MedicineBoston

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