The Journal of Membrane Biology

, Volume 30, Issue 1, pp 381–401 | Cite as

Membrane structural and functional responses to vasopressin in toad bladder

  • William A. Kachadorian
  • James B. Wade
  • Carole C. Uiterwyk
  • Vincent A. DiScala
Article

Summary

Freeze-fracture electronmicroscopy demonstrates that vasopressin stimulation of isolated toad bladder results in a striking morphologic alteration of epithelial membrane structure. This alteration is characterized by the aggregation of intramembranous particles in orderly linear arrays at multiple sites in the luminal membranes of granular cells specifically. The size of these aggregates varies considerably, in terms of area, over a range from 0.5 to 70×10−3 μm2. The median aggregate size is about 10.5×10−3 μm2. Since the extent of vasopressin-associated particle aggregation, in terms of frequency of sites per area of membrane or cumulative area of membrane occupied by them, closely correlates with induced changes in transport function, as measured by osmotic water flow, the aggregates themselves appear to be of physiologic significance in the mechanism of action of vasopressin. This hypothesis is supported by the observations that sites of aggregation occur (a) in response to serosal exposure to hormone specifically, (b) independently of an osmotic gradient, and (c) following stimulation with cyclic adenosine monophosphate.

Keywords

Vasopressin Functional Response Aggregate Size Adenosine Monophosphate Granular Cell 

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Copyright information

© Springer-Verlag New York Inc. 1977

Authors and Affiliations

  • William A. Kachadorian
    • 1
  • James B. Wade
    • 1
  • Carole C. Uiterwyk
    • 1
  • Vincent A. DiScala
    • 1
  1. 1.Renal ServiceU.S. Public Health Service HospitalStaten Island

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