The Journal of Membrane Biology

, Volume 30, Issue 1, pp 197–212 | Cite as

Permeability of barnacle muscle fibers to water and nonelectrolytes

  • Daniel F. Wolff
  • Osvaldo A. Alvarez
  • Fernando F. Vargas
Article
  • 26 Downloads

Summary

The permeability of isolated muscle fibers of the giant barnacleMegabalanus psittacus to water and nonelectrolytes was studied by determining their reflection and permeability coefficients. Reflection coefficients were obtained by comparing the osmotic fluxes produced by a test molecule to that produced by the impermeant sucrose molecule. Permeability coefficients were determined for measurements of tracer uptake.

The results indicate that, in these fibers, nonelectrolyte permeability is closely related to lipid solubility and molecular size.

A value of 2.16×10−12 cm3/sec dyne for the hydraulic conductivity and a value of 10.45×10−4 cm/sec for3HHO permeability coefficient were obtained.

The effect of membrane surface invaginations and clefts on the determination of permeability coefficients is discussed.

Keywords

Lipid Sucrose Muscle Fiber Human Physiology Hydraulic Conductivity 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Birks, R.J., Davey, F.D., 1969. Osmotic responses demonstrating the extracellular character of the sarcoplasmic reticulum.J. Physiol. (London) 202:171Google Scholar
  2. 2.
    Birks, R.J., Davey, D.F., 1972. An analysis of volume changes in the T-tubes of frog skeletal muscles exposed to sucrose.J. Physiol. (London) 222:95Google Scholar
  3. 3.
    Bunch, W., Edwards, G. 1969. The permeation of non-electrolytes through the single barnacle muscle cell.J. Physiol. (London) 202:683Google Scholar
  4. 4.
    Caille, J.P., Hinke, J.A.M. 1973. Evidence for K+ and Cl binding inside muscle from diffusion studies.Can. J. Physiol. Pharmacol. 51:390Google Scholar
  5. 5.
    Caille, J.P., Hinke, J.A.M. 1974. The volume available for diffusion in the muscle fiber.Can. J. Physiol. Pharmacol. 52:814Google Scholar
  6. 6.
    Collander, R. 1954. The permeability of Nitella cells to non-electrolytes.Physiol. Plant. 7:420Google Scholar
  7. 7.
    Crank, J. 1970. The Mathematics of Diffusion. Oxford University Press, LondonGoogle Scholar
  8. 8.
    Dainty, J., House, C.R., 1966. Unstirred layers in frog skin.J. Physiol. (London) 182:66Google Scholar
  9. 9.
    Diamond, J.M. 1966. A rapid method of determining voltage concentration relations across membranes.J. Physiol. (London) 183:83Google Scholar
  10. 10.
    Fenichel, I.R., Horowitz, S.B. 1963. The transport of nonelectrolytes in muscle as a diffusional process in cytoplasm.Acta Physiol. Scand. 60(221):1Google Scholar
  11. 11.
    Gayton, D.C., Hinke, J.A.M. 1968. The location of chloride in single striated muscle fibers of the giant barnacle.Can. J. Physiol. Pharmacol. 46:213Google Scholar
  12. 12.
    Hoyle, G., McNeill, P.A., Selverston, A.I. 1973. Ultrastructure of barnacle giant muscle fibers.J. Cell Biol. 56:74Google Scholar
  13. 13.
    Kedem, O., Katchalsky, A. 1958. Thermodynamic analysis of the permeability of biological membranes to non-electrolytes.Biochim. Biophys. Acta 27:229Google Scholar
  14. 14.
    Keynes, R.D., Rojas, E., Taylor, R.E., Vergara, J. 1973. Calcium and potassium systems of a giant barnacle muscle fiber under membrane potential control.J. Physiol. (London) 229:409Google Scholar
  15. 15.
    Lippe, C. 1969. Urea and thiourea permeability of phospholipid and cholesterol bilayer membranes.J. Mol. Biol. 39:669Google Scholar
  16. 16.
    Paganelli, C.V., Solomon, A.K. 1957. The rate of exchange of tritiated water across the human red cell membrane.J. Gen. Physiol. 41:259Google Scholar
  17. 17.
    Park, C.R., Crafford, O.B., Kono, T. 1968. Mediated (non-active) transport of glucose in mammalian cells and its regulation.J. Gen. Physiol. 52:296sGoogle Scholar
  18. 18.
    Reuben, J.P., Girardier, L., Grundfest, H. 1964. Water transport and cell structure in isolated crayfish muscle fibers.J. Gen. Physiol. 47:1141Google Scholar
  19. 19.
    Smyth, D.H., Wright, E.M. 1966. Streaming potentials in the rat small intestine.J. Physiol. (London) 182:591Google Scholar
  20. 20.
    Solomon, A.K. 1968. Characterization of biological membranes by equivalent pores.J. Gen. Physiol. 51:335sGoogle Scholar
  21. 21.
    Sorenson, A.L. 1971. Water permeability of isolated muscle fibers of a marine crab.J. Gen. Physiol. 58:287Google Scholar
  22. 22.
    Suenson, M., Richmond, D.R., Bassingthwaighte, J.B. 1974. Diffusion of sucrose, sodium and water in ventricular myocardium.Am. J. Physiol. 227:1116Google Scholar
  23. 23.
    Vargas, F.F. 1968. Water flux and electrokinetic phenomena in the squid axon.J. Gen. Physiol. 51:126sGoogle Scholar
  24. 24.
    Vargas, F.F., Johnson, J.A. 1964. An estimate of reflection coefficients for rabbit heart capillaries.J. Gen. Physiol. 47:667Google Scholar
  25. 25.
    Wedner, H.J., Diamond, J.M. 1969. Contributions of unstirred-layer effects to apparent electrokinetic phenomena in the gall-bladder.J. Membrane Biol. 1:92Google Scholar
  26. 26.
    Wright, E.M., Diamond, J.M. 1969. An electric method of measuring non-electrolyte permeability.Proc. R. Soc. London B 172:203Google Scholar
  27. 27.
    Wright, E.M., Diamond, J.M. 1969. Patterns of non-electrolyte permeability.Proc. R. Soc. London B 172:227Google Scholar
  28. 28.
    Wright, E.M., Prather, J.W. 1970. The permeability of the frog choroid plexus to non-electrolytes.J. Membrane Biol. 2:127Google Scholar

Copyright information

© Springer-Verlag New York Inc. 1976

Authors and Affiliations

  • Daniel F. Wolff
    • 1
  • Osvaldo A. Alvarez
    • 1
  • Fernando F. Vargas
    • 1
  1. 1.Department of Biology, Faculty of SciencesUniversity of ChileSantiagoChile

Personalised recommendations