Summary
The possible regulation of Cl− influx inChara by the cytoplasmic Cl− concentration and cytoplasmic pH was investigated using both intact and intracellularly perfused cells. In perfused cells Cl− influx was sensitive to changes in the internal Cl− concentration but only when the concentration was less than 1mm.
In intact cells the metabolic inhibitors, CCCP, DCMU, and oligomycin which inhibit Cl− influx also reduced the cytoplasmic pH. A correlation between ATP concentration and cytoplasmic pH was shown to apply when the ATP concentration was lowered using these inhibitors. The possible relationships between ATP status, cytoplasmic pH, and Cl− influx are discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bostrom, T.E. 1976. Intracellular transport of ions inChara australis. Ph.D. Thesis. University of Sydney, Sydney
Heath, R.L., Hind, G. 1969. The role of Cl− in photosynthesis: II. The effect of Cl− on fluorescence.Biochim. Biophys. Acta 172:290–299
Lin, W., Hanson, J.B. 1974. Phosphate absorption rates and adenosine 5′-triphosphate concentrations in corn root tissue.Plant Physiol. 54:250–256
Raven, J.A., Smith, F.A. 1978. Effect of temperature and external pH on the cytoplasmic pH ofChara corallina.J. Exp. Bot. 29:853–866
Reid, R.J., Walker, N.A. 1983. Adenylate concentrations inChara: Variability, effects of inhibitors and relationship to protoplasmic streaming.Aust. J. Plant Physiol. (in press)
Reid, R.J., Walker, N.A. 1984. Energetics of Cl− active transport inChara. J. Membrane Biol. (in press)
Sanders, D. 1978. Regulation of ion transport in Characean cells. Ph. D. Thesis. University of Cambridge, Cambridge
Sanders, D. 1980a. Control of Cl− influx inChara by cytoplasmic Cl− concentration.J. Membrane Biol. 52:51–60
Sanders, D. 1980b. The mechanism of Cl− transport at the plasma membrane ofChara corallina: I. Cotransport with H+.J. Membrane Biol. 53:129–142
Sanders, D. 1981. Physiological control of chloride transport inChara corallina: II. The role of chloride as a vacuolar osmoticum.Plant Physiol 68:401–406
Sanders, D., Hansen, U.-P. 1981. Mecyanism of Cl− transport at the plasma membrane ofChara corallina: II. Transinhibition and the determination of H+/Cl− binding order from a reaction kinetic model.J. Membrane Biol. 58:139–153
Smith, F.A. 1980. Comparison of the effects of ammonia and methylamine on chloride transport and intracellular pH inChara corallina. J. Exp. Bot.31:597–606
Smith, F.A., MacRobbie, E.A.C. 1981. Comparison of cytoplasmic pH and Cl− influx in cells ofChara corallina following “Cl− starvation”.J. Exp. Biol. 32:827–835
Smith, F.A., Raven, J.A. 1979. Intracellular pH and its regulation.Annu. Rev. Plant Physiol. 30:289–311
Smith, P.T., Walker, N.A. 1981. Studies on the perfused plasmalemma ofChara corallina: I. Current — voltage curves. ATP and potassium dependence.J. Membrane Biol. 60:223–236
Walker, N.A., Smith, F.A. 1975. Intracellular pH inChara corallina measured by DMO distribution.Plant Sci. Lett. 4:125–132
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Reid, R.J., Walker, N.A. Control of Cl− influx inChara by internal pH. J. Membrain Biol. 78, 157–162 (1984). https://doi.org/10.1007/BF01869202
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01869202