The Journal of Membrane Biology

, Volume 78, Issue 2, pp 119–127 | Cite as

Ion permeability of rabbit intestinal brush border membrane vesicles

  • Robert D. Gunther
  • Richard E. Schell
  • Ernest M. Wright
Articles

Summary

The ion permeability of rabbit jejunal brush border membrane vesicles was studied by measuring unidirectional fluxes with radioactive tracers and bi-ionic diffusion potentials with the potential-sensitive fluorescent dye, diS−C3-(5). Tracer measurements provide estimates of the absolute magnitudes of permeability coefficients, while fluorescence measurements provide estimates of relative and absolute ion permeabilities. The magnitudes of the permeability coefficients for Na+, K+, Rb+, and Br were approximately 5 nanoliters/(mg protein × sec) or 10−5 cm/sec as determined by radioactive tracer measurements. The apparent selectivity sequence, relative to Na+, as determined by bi-ionic potential measurements was: F, isetheionate, gluconate, choline (<0.1)<Na+(1.0)<Cl(1.5)=NO 3 (1.5)<Br(2.3)<K+(2.4)<Rb+(2.5)<Cs+(2.6)<Li+(3.9) <NH 4 +(12)<I(40). The origin of this selectivity sequence and its relationship to the ion permeability of the brush border membrane in the intact epithelium are discussed.

Key Words

ion permeability potential-sensitive cyanine dye intestinal brush border vesicles 

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Copyright information

© Springer-Verlag New York Inc. 1984

Authors and Affiliations

  • Robert D. Gunther
    • 1
  • Richard E. Schell
    • 1
  • Ernest M. Wright
    • 1
  1. 1.Department of PhysiologyUCLA School of MedicineLos Angeles

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