The Journal of Membrane Biology

, Volume 51, Issue 2, pp 161–184 | Cite as

Coupled transepithelial sodium and potassium transport across isolated frog skin: Effect of ouabain, amiloride and the polyene antibiotic filipin

  • Robert Nielsen
Articles

Summary

Addition of the polyene antibiotic filipin (50 μm) to the outside bathing solution (OBS) of the isolated frog skin resulted in a highly significant active outward transport of K+ because filipinper se increases the nonspecific Na+ and K+ permeability of the outward facing membrane. The K+ transport was calculated from the chemically determined changes in K+ concentrations in the solution bathing the two sides of the skin. The active transepithelial K+ transport required the presence of Na+ in the OBS, but not in the inside bathing solution (IBS), and it was inhibited by the Na+, K+-ATPase inhibitor ouabain. The addition of Ba++ to the IBS in the presence of filipin in the OBS resulted in an activation of the transepithelial K+ transport and in an inhibition of the active Na+ transport. This is in agreement with the notion that Ba++ decreases the passive K+ permeability of the inward facing membrane. In the presence of amiloride (which blocks the specific Na permeability of the outward facing membrane) and Ba++ there was a good correlation between the active Na+ and K+ transport. It is concluded that the active transepithelial K+ transport is carried out by a coupled electrogenic Na−K pump, and it is suggested that the pump ratio (Na/K) is 1.5.

Keywords

Sodium Potassium Human Physiology Ouabain Amiloride 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bakhteeva, V.T., Natochin, Y.V. 1975. The passive and active elements of potassium secretion system in the frog skin.Lechenow Physiol. J. USSR 61:1242Google Scholar
  2. Biber, T.U.L., Aceves, J., Mandel, J.L. 1972. Potassium uptake across serosal surface of isolated frog skin epithelium.Am. J. Physiol. 222:1366Google Scholar
  3. Cala, P.M., Cogswell, N., Mandel, L.J. 1978. Binding of3H ouabain to split frog skin.J. Gen. Physiol. 71:347Google Scholar
  4. Candia, O.A., Zadunaisky, J.A. 1972. Potassium flux and sodium transport in the isolated frog skin.Biochim. Biophys. Acta 255:517Google Scholar
  5. Curran, P.F., Cereijido, M. 1965. K fluxes in frog skin.J. Gen. Physiol. 48:1011Google Scholar
  6. Cuthbert, A.W., Shum, W.K. 1974. Binding of amiloride to sodium channels in frog skin.Mol. Pharmacol. 10:880Google Scholar
  7. DeLong, J., Civan, M.M. 1978. Dissociation of cellular K+ accumulation from net Na+ transport by toad urinary bladder.J. Membrane Biol. 42:19Google Scholar
  8. Essig, A., Leaf, A. 1963. The role of potassium in active transport of sodium by the toad bladder.J. Gen. Physiol. 46:505Google Scholar
  9. Finn, A.L., Nellans, H. 1972. The kinetics and distribution of potassium in the toad bladder.J. Membrane Biol. 8:189Google Scholar
  10. Frazier, L.W., Vanatta, J.C. 1971. Relationship of Na transport and K excretion by the urinary bladder ofBufo marinus.Proc. Soc. Exp. Biol. Med. 136:235Google Scholar
  11. Frizzell, R.A., Turnheim, K. 1978. Ion transport by rabbit colon: Unidirectional sodium influx and the effects of amphotericin B and amiloride.J. Membrane Biol. 40:193Google Scholar
  12. Giebisch, G., Sullivan, L.P., Whittenbury, G. 1973. Relationship between tubular net sodium reabsorption and pertibular potassium uptake in the perfusedNecturus kidney.J. Physiol. (London) 230:51Google Scholar
  13. Harris, E.J., Burn, C.P. 1949. The transfer of sodium and potassium ions between muscle and the surrounding medium.Trans. Faraday Soc. 45:508Google Scholar
  14. Henderson, E.C. 1974. Strophanthidin sensitive electrogenic mechanisms in frog sartorius muscles exposed to barium.Pfluegers Arch. 350:81Google Scholar
  15. Hermsmeyer, K., Sperelakis, N. 1970. Decrease in K+ conductance and depolarization of frog cardiac muscle produced by Ba++.Am. J. Physiol. 219:1108Google Scholar
  16. Huf, E.C., Wills, J. 1951. Influence of some inorganic cations on active salt and water uptake by isolated frog skin.Am. J. Physiol. 167:255Google Scholar
  17. Kawada, J., Taylor, R.E., Barker, S.B. 1969. Measurement of Na−K-ATPase in the separated epidermis ofRana catesbeiana frogs and tadpoles.Comp. Biochem. Physiol. 30:965Google Scholar
  18. Koefoed-Johnsen, V. 1957. The effect of g-strophantin (ouabain) on the active transport of sodium through the isolated frog skin.Acta Physiol. Scand (Suppl.)145:87Google Scholar
  19. Koefoed-Johnsen, V., Ussing, H.H. 1958. The nature of the frog skin potential.Acta Physiol. Scand 42:298Google Scholar
  20. Lewis, S.A., Wills, N.K., Eaton, D.C. 1978. Basolateral membrane potential of a tight epithelium: Ionic diffusion and electrogenic pumps.J. Membrane Biol. 41:117Google Scholar
  21. Nagel, W. 1978. Ba++ decreasesC K in frog skin.Fed. Proc. 37:569Google Scholar
  22. Nellans, H.N., Schultz, S.C. 1976. Relations among transepithelial sodium transport, potassium exchange, and cell volume in rabbit ileum.J. Gen. Physiol. 68:441Google Scholar
  23. Nielsen, R. 1971. Effect of amphotericin B on the frog skinin vitro. Evidence for outwards active potassium transport across the epithelium.Acta Physiol. Scand. 83:106Google Scholar
  24. Nielsen, R. 1972. The effect of polyene antibiotics on the aldosterone induced changes in sodium transport across the isolated frog skin.J. Steroid Biochem. 3:121Google Scholar
  25. Nielsen, R. 1977. Effect of the polyene antibiotic filipin on the permeability of the inward-and the outward-facing membrane of the isolated frog skin.Acta Physiol. Scand. 99:399Google Scholar
  26. Nielsen, R. 1978. Effect of the polyene antibiotic filipin and the calcium ionophore A23187 on sodium transport in isolated frog skin.J. Membrane Biol. Special Issue: 331Google Scholar
  27. Nielsen, R., Tomlinson, R.W.S. 1970. The effect of amiloride on sodium transport in the normal and moulting frog skin.Acta Physiol. Scand. 42:298Google Scholar
  28. Robinson, B.A., Macknight, A.D.C. 1976. Relationships between serosal medium potassium concentration and sodium transport in toad urinary bladder. III. Exchangeability of epithelial cellular potassium.J. Membrane Biol. 26:269Google Scholar
  29. Thomas, R.C. 1972. Electrogenic sodium pump in nerve and muscle cells.Physiol. Rev. 52:563Google Scholar
  30. Ussing, H.H. 1978. Interpretation of tracer fluxes.In: Membrane Transport in Biology. G. Giebisch, D.C. Tosteson, and H.H. Ussing, editors. Vol. 1, p. 115. Springer-Verlag, BerlinGoogle Scholar
  31. Ussing, H.H., Zerahn, K. 1951. Active transport of sodium as the source of electric current in the short-circuited isolated frog skin.Acta Physiol. Scand. 23:110Google Scholar
  32. Valenzeno, D.P., Hoshiko, T. 1977. Potassium reaccumulation by isolated frog epidermis.Biochim. Biophys. Acta 470:273Google Scholar

Copyright information

© Springer-Verlag New York Inc. 1979

Authors and Affiliations

  • Robert Nielsen
    • 1
  1. 1.Institute of Biological Chemistry ACopenhagen ØDenmark

Personalised recommendations