On the mechanism of electrical coupling between cells of earlyXenopus embryos
- 23 Downloads
The mechanism of electrical coupling between cells of earlyXenopus embryos has been studied by examination of the nonjunctional membrane resistances and capacitances as a function of cleavage stage, the junctional and nonjunctional membrane resistances as functions of time during the first cleavage, and the electrical properties of the primitive blastocoel. The changes in membrane resitances and capacitances during the first two cleavages may be completely explained by the addition of new membrane, identical in specific resistance and capacitance to the original membrane, at a constant rate to furrows which are electrically connected to the perivitelline space. Microelectrode recording from the primitive blastocoel indicates that there is no electrical difference detectable between it and the perivitelline space. These results are discussed in the context of current theories of the mechanism of intercellular electrotonic coupling.
KeywordsHuman Physiology Electrical Property Current Theory Membrane Resistance Cleavage Stage
Unable to display preview. Download preview PDF.
- DiCaprio, R.A., French, A.S., Sanders, E.J. 1974. Dynamic properties of electrotonic coupling between cells of earlyXenopus embryos.Biophys. J. 14:387Google Scholar
- Gilula, N.B., Reeves, O.R., Steinbach, A. 1972. Metabolic coupling, ionic coupling and cell contacts.Nature (London) 235:262Google Scholar
- Hamburger, V. 1960. A Manual of Experimental Embryology, University of Chicago Press, ChicagoGoogle Scholar
- Ito, S., Sato, E., Loewenstein, W.R. 1974a. Studies on the formation of a permeable cell membrane junction. I. Coupling under various conditions of membrane contact. Effects of Colchicine, Cytochalasin-B, Dinitrophenol.J. Membrane Biol. 19:305Google Scholar
- Ito, S., Sato, E., Loewenstein, W.R. 1974b. Studies on the formation of a permeable cell membrane junction. II. Evolving junctional conductance and junctional insulation.J. Membrane Biol. 19:339Google Scholar
- Loewenstein, W.R. 1968. Communication through cell junctions. Implications in growth control and differentiation.Dev. Biol. 19(Suppl. 2):151Google Scholar
- Sanders, E.J., Zalik, S.E. 1972. The blastomere periphery ofXenopus laevis with special reference to intercellular relationships.Wilhelm Roux' Arch. Entwicklungsmech. Org. 171:181Google Scholar