Summary
Paired toad urinary bladders were prepared without or with an osmotic gradient (175 mosm) across them, stimulated for 2.5 (n=6), 5 (n=6), 30 (n=6) or 60 (n=6) min with ADH (20 mU/ml), and studied by freeze-fracture electron microscopy. Water permeability at these times was assessed in additional bladders (n=6 for each case) after tissue fixation according to the technique of Eggena. After both 60 and 30 min of ADH stimulation, the presence of a gradient compared with the absence of one was associated with fewer aggregates (242±35vs. 382±14 ×235 μm−2 at 60 min,P<0.01; 279±36vs. 470±51 ×235 μm−2 at 30 min,P<0.01) and lower water permeability (8.4±1.1vs. 18.8±1.8μg×min−1×cm−1 ×mosm −1 at60min,P<0.005; 9.2±1.0vs. 22.0±2.1 μg ×min−1×cm−2×mosm −1 at 30 min,P<0.001). In addition, with a gradient both maximum water permeability and maximum aggregate frequency were reached nearly together; a similar correspondence occurred without a gradient. We conclude that in the presence of an osmotic gradient both the ADH-associated aggregates and the water permeability response to ADH are prevented from reaching the higher levels observed in bladders not exposed to a gradient.
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References
Bentley, P.J. 1958. The effect of neurophypophysial extracts on water transfer across the wall of the isolated urinary bladder of the toadBufo marinus.J. Endocrinol. 17:201
Bourguet, J., Chevalier, J., Hugon, J.S. 1976. Alterations in membrane-associated particle distribution during antidiuretic challenge in frog urinary bladder epithelium.Biophys. J. 16:627
Chevalier, J., Bourguet, J., Hugon, J.S. 1974. Membrane associated particle distribution in frog urinary bladder epithelium at rest and after oxytocin treatment.Cell Tissue Res. 152:129
Dratwa, M., Tisher, C.C., Somer, J.R., Croker, B.R., Jr. 1979. Intramembranous particle aggregation in toad urinary bladder after vasopressin stimulation.Lab. Invest. 40(1):46
Edelman I.S., Peterson, M.J., Gulyassy, P.F. 1964. Kinetic analysis of the antidiuretic action of vasopressin and adenosine-3′, 5′ monophosphate.J. Clin. Invest. 43:2185
Eggena, P. 1972. Osmotic regulation of toad bladder responsiveness to neurohypophyseal hormones.J. Gen. Phys. 60:665
Eggena, P., Christakis, D., Deppisch, L. 1975. Effect of hypotonicity on cyclic adenosine monophosphate formation and action in vasopressin target cells.Kidney Int. 7:161
Kachadorian, W.A., Casey, C., DiScala, V.A. 1978. Time course of ADH-induced intramembranous particle aggregation in toad urinary bladder.Am. J. Physiol. 234 (6:F461
Kachadorian, W.A., Levine, S.D., Wade, J.B., DiScala, V.A., Hays, R.M. 1977. Relationship of aggregated intramembranous particles to water permeability in vasopressin treated toad urinary bladder.J. Clin. Invest. 59:576
Kachadorian, W.A., Wade, J.B., DiScala, V.A. 1975. Vasopressin: Induced structural change in toad bladder luminal membrane.Science 190:67
Kachadorian, W.A., Wade, J.B., Uiterwyk, C.C., DiScala, V.A. 1977. Membrane Structural and functional responses to vasopressin in toad bladder.J. Membrane Biol. 30:381
Levine, S.D., Kachadorian, W.A., Schlondorff, D. 1979. Effect of hydrazine on vasopressin-stimulated water flow, membrane particle aggregates, and kinase activity in toad bladder.Clin. Res. 27:422A (Abstr.)
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Ellis, S.J., Kachadorian, W.A. & DiScala, V.A. Effect of osmotic gradient on ADH-induced intramembranous particle aggregates in toad bladder. J. Membrain Biol. 52, 181–184 (1980). https://doi.org/10.1007/BF01869124
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DOI: https://doi.org/10.1007/BF01869124