Skip to main content
SpringerLink
Log in

Preparation of multilayers containing chlorophylla and/or phosphatidylcholine and chemical stability of chlorophylla molecules in the multilayers

  • Published:
The Journal of Membrane Biology Aims and scope Submit manuscript

Summary

The Langmuir-Blodgett films consisting of mixed monolayers containing chlorophylla (Chl-a) and/or phosphatidylcholine with various molar ratios could be well prepared on glass plates previously covered with three monolayers of cadmium eicosanate. Thin-layer and high-performance liquid chromatographic examinations revealed that the photostability of Chl-a molecules in various media is in the following order: the mixed mono- or multilayers >pure Chl-a mono- or multilayers≫organic solvents.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Aoshima, R., Iriyama, K., Asai, H. 1973. High sensitivity fluorophotometer using photon counting.Applied Optics 12:2748

    Google Scholar 

  2. Aoshima, R., Iriyama, K., Asai, H. 1975. Fluorescence properties of chlorophylla and monomolecular films at the air-water interface.Biochim. Biophys. Acta 406:362

    PubMed  Google Scholar 

  3. Bellamy, W.D., Gaines, G.L., Jr., Tweet, A.G. 1963. Preparation and properties of monomolecular films of chlorophylla and pheophytina.J. Chem. Phys. 39:2528

    Google Scholar 

  4. Comar, C.L., Zscheile, F.P. 1942. Analysis of plant extracts for chlorophylla andb by a photoelectric spectrophotometric method.Plant Physiol. 17:198

    Google Scholar 

  5. Iriyama, K. 1975. The method for preparation of mono- and multilayers containing egg phosphatidylcholine.Bull. Res. Inst. Pol. Tex. (Yokohama, Japan)106:11

    Google Scholar 

  6. Iriyama, K. 1978. A rapid and convenient method for purification and isolation of chlorophyll-a fromPorphyra yezoensis.Biochem. Biophys. Res. Commun. 83:501

    PubMed  Google Scholar 

  7. Iriyama, K. 1979. Chemical stability of chlorophylla in benzene solutions and in monolayers at a water-air interface.J. Colloid Interface Sci. 68:381

    Google Scholar 

  8. Iriyama, K. 1979. Methods for preparing chlorophyll-a multilayers on glass plates.Photochem. Photobiol. 29:633

    Google Scholar 

  9. Iriyama, K., Shiraki, M., Yoshiura, M. 1979. An improved method for extraction, partial purification, separation and isolation of chlorophyll from spinach leaves.J. Liquid Chromatogr. 2:255

    Google Scholar 

  10. Iriyama, K., Yoshiura, M., Shiraki, M. 1978. Micro-method for the qualitative and quantitative analysis of photosynthetic pigments using high-performance liquid chromatography.J. Chromatogr. 154:302

    Google Scholar 

  11. Ke, B. 1966. Some properties of chlorophyll monolayers and crystalline chlorophyll.In: The Chlorophylls. L.P. Vernon and G.R. Seely, editors. p. 253. Academic Press, New York

    Google Scholar 

  12. Kunn, H., Möbius, D., Bücher, H. 1973. Spectroscopy of monolayer assemblies.In; Techniques of Chemistry. A. Weissberger and B.W. Rossiter, editors. p. 577. John Wiley & Sons, New York

    Google Scholar 

  13. Leblanc, R.M., Chapados, C. 1977. Aggregation of chlorophylls in monolayer. II. Chlorophyll-dioxane interaction.Biophys. Chem. 6:77

    Google Scholar 

  14. Schwertner, H.A., Biale, J.B. 1973. Lipid composition of plant mitochondria and of chloroplasts.J. Lipid Res. 14:235

    PubMed  Google Scholar 

  15. Shimojo, T., Ohnishi, T. 1967. Studies on membrane model. I. Surface pressure and surface potential of pure phospholipid monolayers.J. Biochem. 61:89

    PubMed  Google Scholar 

  16. Shiraki, M., Yoshiura, M., Iriyama, K. 1978. Rapid and easy separation of chlorophylls, their derivatives, and plant yellow pigments by thin-layer chromatography.Chem. Lett. (Tokyo) p. 103

  17. Stillwell, W., Tien, H.T. 1977. Protection of chlorophyll by phospholipids from photooxidation.Biochem. Biophys. Res. Commun. 75:232

    Google Scholar 

  18. Strain, H.H., Svec, W.A. 1966. Extraction, separation, estimation, and isolation of chlorophylls.In: The Chlorophylls. L.P. Vernon and G.R. Seely, editors. p. 21. Academic Press. New York

    Google Scholar 

  19. Yoshiura, M., Iriyama, K., Shiraki, M. 1978. High-performance liquid chromatography of chlorophylls and some of their derivatives.Chem. Lett. (Tokyo) p. 281

Download references

Author information

Authors and Affiliations

  1. Research Institute for Polymers and Textiles, 1-1-4 Yatabe-Higashi, 305, Tsukuba, Ibaraki Pref., Japan

    Keiji Iriyama

Authors
  1. Keiji Iriyama
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Iriyama, K. Preparation of multilayers containing chlorophylla and/or phosphatidylcholine and chemical stability of chlorophylla molecules in the multilayers. J. Membrain Biol. 52, 115–120 (1980). https://doi.org/10.1007/BF01869116

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01869116

Keywords

Search

Navigation