The Journal of Membrane Biology

, Volume 114, Issue 2, pp 175–188 | Cite as

Patch-clamp profile of ion channels in resting murine B lymphocytes

  • Frances V. McCann
  • David C. McCarthy
  • Randolph J. Noelle
Articles

Summary

Patch-clamp studies of single ion channel currents in freshly isolated murine B lymphocytes are characterized here according to their respective unitary conductances, ion selectivities, regulatory factors, distributions and kinetic behavior. The most prevalent ion channel in murine B lymphocytes is a large conductance (348 pS) nonselective anion channel. This report characterizes additional conductances including: two chloride channels (40 and 128 pS), a calcium-activated potassium channel (93 pS), and an outwardly rectifying potassium channel which displays two distinct conductances (18 and 30 pS). Like the anion channel, both chloride channels exhibit little activity in the cellattached patch configuration. The kinetic behavior of all of these channels is complex, with variable periods of bursting and flickering activity interspersed between prolonged closed/open intervals (dwell times). It is likely that some of these channels play an important role in the signal transduction of B cell activation.

Key Words

anion channel potassium channel chloride channel patch clamp single-channel recording murine B lymphocytes 

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Copyright information

© Springer-Verlag New York Inc 1990

Authors and Affiliations

  • Frances V. McCann
    • 1
  • David C. McCarthy
    • 1
  • Randolph J. Noelle
    • 2
  1. 1.Department of PhysiologyDartmouth Medical SchoolHanover
  2. 2.Department of MicrobiologyDartmouth Medical SchoolHanover

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