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Control of Cl influx inChara by cytoplasmic Cl− concentration

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Summary

Cl− influx into cells ofChara corallina is shown to be stimulated by a factor of 2 to 4 by starvation of Cl−. The time constant for the induction of this effect is about 4.0 ksec and that for its decay when Cl− is reprovided, 1.7 ksec. Intracellular perfusion of tonoplast-free cells with solutions of varying Cl− concentration shows that Cl− influx can be controlled directly by the concentration of Cl− at the inside of the plasma membrane. Both the time course for the initial stages of induction of the starvation-stimulated flux and its absolute magnitude can be accounted for by assuming cytoplasmic Cl− concentration to be the only intracellular condition to change during Cl− starvation. The existence of a feedback loop between cytoplasmic Cl− and Cl− influx provides an alternative explanation to observations previously used in support of a Cl−/OH− exchange hypothesis (F.A. Smith, 1972,New Phytol. 71:595).

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  1. Dale Sanders

    Present address: Physiology Department, Yale Medical School, 333 Cedar Street, 06510, New Haven, Connecticut

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  1. Botany School, Downing Street, CB2 3EA, Cambridge, England

    Dale Sanders

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Sanders, D. Control of Cl influx inChara by cytoplasmic Cl− concentration. J. Membrain Biol. 52, 51–60 (1980). https://doi.org/10.1007/BF01869005

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  • DOI: https://doi.org/10.1007/BF01869005

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