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The Journal of Membrane Biology

, Volume 57, Issue 2, pp 119–131 | Cite as

Role of external potassium in the calcium-induced potassium efflux from human red blood cell ghosts

  • Agnes Heinz
  • Hermann Passow
Articles

Summary

The exposure of red cell ghosts to external Ca++ and K+ leads to a rapid net K+ efflux. Preincubation of the ghosts for various lengths of time in the absence of K+ in the external medium prior to a challenge with maximally effective concentrations of Ca++ and K+ renders the ghosts unresponsive to that challenge with a half-time of about 7–10 min. Preincubation at a range of K+ concentrations for a fixed length of time (60 min) prior to the challenge revealed that K+ concentrations of about 500 μm or more suffice to maintain the K+ channel in a maximally responsive state for at least 60 min. These K+ concentrations are considerably lower than the K+ concentrations required to make the responsive channel respond with a maximal rate of K+ efflux. Thus, external K+ is not only necessary to induce the permeability change but also to maintain the transport system in a functional state.

The presence of Mg++ or ethylenediamine-tetraacetic acid (EDTA) in the K+-free preincubation media preserves the responsiveness to a challenge with Ca++ plus K+. In contrast to external K+, the presence of external Ca++ does not reduce but rather enhances the loss of responsiveness. An excess of EDTA prevents the effects of Ca++ while washes with EDTA after exposure to Ca++ do not reverse them.

In red cell ghosts that contain Ca++ buffers, the transition from a responsive to a nonresponsive state incubation in the absence of external K+ is enhanced. The effects of incubation in the presence of Ca++ in K+-free media are reversed; external Ca++ now reduces the rate at which the responsiveness is lost. The loss of responsiveness after incubation in K+-free media prior to a challenge with external K+ and internal Ca++ does also take place when K+-efflux from red cell ghosts is measured by means of42K+ into media that have the same K+ concentrations as the ghost interior. This confirms that the effects of K+-free incubation are due to the modification of the K+-selective channel rather than to an inhibition of diffusive Cl-efflux.

Keywords

EDTA Effective Concentration Free Media External Medium Permeability Change 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviation used in text

TRIS

Tris (hydroxymethyl) aminomethan

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Copyright information

© Springer-Verlag New York Inc. 1980

Authors and Affiliations

  • Agnes Heinz
    • 1
  • Hermann Passow
    • 1
  1. 1.Max-Planck-Institut für BiophysikFrankfurt am MainWest Germany

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