Control of the uptake of amino acids by serum in chick embryo cells, untransformed or transformed with rous sarcoma virus
- 21 Downloads
Forty to fifty minutes after removal of serum, the net total uptake of amino acids in growing secondary cultures of normal or virus-transformed chick embryo cells, stopped or proceeded only at a highly reduced rate. In both normal and transformed cells, theinitial (0–40 min) rate of the above uptake was the same in the absence of serum as in its presence. The initial rate of the total uptake of amino acids in growing transformed cells was about the same as in growing normal cells. Neither in the normal nor in the transformed cells was the rate of the total uptake of amino acids reduced by cell confluence alone. In highly dense, hyperconfluent cultures of normal cells in which cell growth was arrested, the rate of uptake in the absence or in the presence of serum was four- to fivefold lower than the rate obtained in growing normal cells under similar conditions; in the absence of serum, the net uptake stopped after 40 min in the hyperconfluent cultures as well. It appears that cells growing in tissue culture require a serum factor for maintenance of the required high rates of uptake of amino acids and that the inhibition of growth at high cell densities is a result of depletion of this factor from serum, or the inability of the cells in a dense culture to respond to the factor. A serum factor is apparently also required for maintenance of the reduced rates of uptake of amino acids observed in hyperconfluent cultures.
KeywordsCell Growth Tissue Culture Sarcoma Cell Density Human Physiology
Unable to display preview. Download preview PDF.
- 3.Bhargava, P.M., Allin, E.P., Montagnier, L. 1976. Uptake of amino acids and thymidine during the first cell cycle of synchronized hamster cells.J. Membrane Biol. 26:1Google Scholar
- 4.Bhargava, P.M., Siddiqui, M.A., Kumar, G.K., Prasad, K.S.N. 1975. Effect of cell concentration on the uptake of amino acids by rat liver parenchymal cells in suspension.J. Membrane Biol. 22:357Google Scholar
- 5.Bhargava, P.M., Szafarz, D., Bornecque, C.A., Zajdela, F. 1976. A comparison of the ability of normal liver, a premalignant liver, a solid hepatoma and the Zajdela ascitic hepatoma, to take up amino acidsin vitro.J. Membrane Biol. 26:31Google Scholar
- 7.Burger, M.M. 1971. Cell surfaces in neoplastic transformation.Curr. Top. Cell. Regul. 3:135Google Scholar
- 8.Christensen, H.N., Rothwell, J.T., Sears, R.A., Streicher, J.A. 1948. Association between rapid growth and elevated cell concentrations of amino-acids. II. In regenerating liver after partial hepatectomy in the rat.J. Biol. Chem. 175:101Google Scholar
- 13.Cunningham, D.D., Pardee, A.B. 1971. Transport and phospholipid change related to control of growth in 3T3 cells.In: Growth Control in Cell Cultures. G.E.W. Wolstenholme and J. Knight, editors. p. 207. Churchill Livingstone, LondonGoogle Scholar
- 16.Golde, A., Villaudy, J. 1972. The effect of ageing and cell density on the infection and the morphological conversionin vitro by Rous sarcoma virus of chick embryo fibroblasts.In: The Biology of Oncogenic Viruses, Proc. 2nd Lepetit Colloquium, Paris, 1972. L. Silvestri, editor. p. 124. North-Holland Publ. Co., AmsterdamGoogle Scholar
- 23.Hershko, A., Mamont, P., Shields, R., Tomkims, G.M. 1971. Hypothesis relating growth regulation in mammalian cells to stringent controls in bacteria.Nature, New Biol. 232:206Google Scholar
- 30.Temin, H.M., Pierson, R.W., Jr., Dulak, J.C. 1972. The role of serum in the control of multiplication of avian and mammalian cells in culture.In: Growth, Nutrition and Metabolism of Cells in Culture. G.H. Rothblat and J.J. Cristofalo, editors. p. 50. Academic Press, New York & LondonGoogle Scholar
- 33.Waller, J.M., Kirsten, W.M. 1970. Density-dependent inhibition of protein synthesis in normal and virus-transformed cells.Virchows Arch. 6:183Google Scholar
- 36.Whitney, R.B., Sutherland, R.M. 1973. Effects of chelating agents on the initial interaction of phytohaemagglutinin with lymphocytes and the subsequent stimulation of aminoacid uptake.Biochim. Biophys. Acta 228:790Google Scholar