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Mechanism of Cl secretion in canine trachea: Changes in intracellular chloride activity with secretion

The Journal of Membrane Biology volume 81, pages 1–8 (1984)Cite this article

Summary

Cl-sensitive microelectrodes were employed to investigate the mechanism of Cl secretion by canine tracheal epithelium. In control tissues with a mean calculated short-circuit current (I sc) of 18.1 μA/cm2, the intracellular Cl activity (a iCl ) was 47.2mm. This value is 30.1mm (or 27.0 mV) above the electrochemical equilibrium for Cl across the apical membrane. Epinephrine, which stimulates Cl secretion, increased the calculatedI sc to 160 μA/cm2 and decreaseda iCl to 32.2mm, a value only 11.2mm (or 10.9 mV) above equilibrium for the apical membrane. These results indicate a secretagogue induced decrease in the impedance to Cl exit from the cell via the apical membrane. From these and prior measurements we calculate that epinephrine-induced Cl efflux from the cell can occur by simple diffusion across the apical membrane. Further implications of these calculations are also discussed.

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  1. Departments of Pharmacological and Physiological Sciences and of Medicine, The University of Chicago, 60637, Chicago, Illinois

    Stephen R. Shorofsky, Michael Field & Harry A. Fozzard

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  1. Stephen R. Shorofsky
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  2. Michael Field
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  3. Harry A. Fozzard
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Shorofsky, S.R., Field, M. & Fozzard, H.A. Mechanism of Cl secretion in canine trachea: Changes in intracellular chloride activity with secretion. J. Membrain Biol. 81, 1–8 (1984). https://doi.org/10.1007/BF01868804

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  • DOI: https://doi.org/10.1007/BF01868804

Key Words

  • intracellular Cl activity
  • Cl transport
  • tracheal epithelium
  • Cl permeability
  • epinephrine
  • equivalent circuit analysis