Summary
The time course of the current following a voltage jump, which is applied to monoglyceride bilayers in the presence of valinomycin, shows two relaxation times. This is basically in agreement with a simple carrier model which has been described in full detail formerly. Relaxation times and amplitudes allow a calculation of the rate constants of the transport model. The presented data supplement an analysis which was hitherto based only on the slower relaxation process and on information derived from the nonlinearity of currentvoltage characteristics. The additional resolution of the faster relaxation time allowed an approximate determination of the voltage dependence of the translocation rate constant for the carrier-ion-complex and provided evidence for a small voltage dependence of the interfacial reaction. The dependence of the relaxation parameters on the ion concentration in the aqueous phase was interpreted assuming a saturation of the ion concentration at the reaction plane at high bulk concentrations.
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References
Benz, R., Stark, G. 1975. Kinetics of macrotetrolide-induced ion transport across lipid bilayer membranes.Biochim. Biophys. Acta 382: 27
Benz, R., Stark, G., Janko, K., Läuger, P. 1973. Valinomycin-mediated ion transport through neutral lipid membranes: Influence of hydrocarbon chain length and temperature.J. Membrane Biol. 14: 339
Feldberg, S.W., Kissel, G. 1975. Charge pulse studies of transport phenomena in bilayer membranes.J. Membrane Biol. 20: 269
Grell, E., Funck, Th. 1973. Dynamic properties and membrane activity of ion specific antibiotics.J. Supramol. Struct. 1: 307
Hall, J.E., Mead, C.A., Szabo, G. 1973. A barrier model for current flow in lipid bilayer membranes.J. Membrane Biol. 11: 75
Hladky, S.B. 1974. The energy barriers to ion transport by nonactin across thin lipid membranes.Biochim. Biophys. Acta 352: 71
Hladky, S.B. 1975. Tests of the carrier model for ion transport by nonactin and trinactin.Biochim. Biophys. Acta 375: 327
Hsu, M., Chan, I.S. 1973. Nuclear magnetic resonance studies of the interaction of valinomycin with unsonicated lecithin bilayers.Biochemistry 12: 3872
Krasne, S., Eisenman, G., Szabo, G. 1971. Freezing and melting of bilayers and the mode of action of nonactin, valinomycin and gramicidin.Science 174: 412
Laprade, R., Ciani, S.M., Eisenman, G., Szabo, G. 1974. The kinetics of carrier-mediated ion permeation in lipid bilayers and its theoretical interpretation.In: Membranes—A Series of Advances. G. Eisenman, editor, Vol. 3. M. Dekker, New York (in press)
Läuger, P., Stark, G. 1970. Kinetics of carrier-mediated ion transport across lipid bilayer membranes.Biochim. Biophys. Acta 211: 458
Liberman, E.A., Topaly, V.P. 1968. Selective transport of ions through bimolecular phospholipid membranes.Biochim. Biophys. Acta 163: 125
Stark, G. 1973. Rectification phenomena in carrier-mediated ion transport.Biochim. Biophys. Acta 298: 323
Stark, G., Benz, R. 1971. The transport of potassium through lipid bilayer membranes by the neutral carriers valinomycin and monactin.J. Membrane Biol. 5: 133
Stark, G., Ketterer, B., Benz, R., Läuger, P. 1971. The rate constants of valinomycin-mediated ion transport through thin lipid membranes.Biophys. J. 11: 981
Szabo, G., Eisenman, G., Ciani, S. 1969. The effects of the macrotetralide actin antibiotics on the electrical properties of phospholipid bilayer membranes.J. Membrane Biol. 1: 346
Tosteson, D.C., Andreoli, T.E., Tieffenberg, M., Cook, P. 1968. The effects of macrocyclic compounds on cation transport in sheep red cells and thin and thick lipid membranes.J. Gen. Physiol. 51: 373
Zwolinsky, B.J., Eyring, H., Reese, C.E. 1949. Diffusion and membrane permeability.J. Phys. Colloid Chem. 53: 1426
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Knoll, W., Stark, G. An extended kinetic analysis of valinomycin-induced Rb-transport through monoglyceride membranes. J. Membrain Biol. 25, 249–270 (1975). https://doi.org/10.1007/BF01868578
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DOI: https://doi.org/10.1007/BF01868578