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The Journal of Membrane Biology

, Volume 25, Issue 1, pp 115–139 | Cite as

Electrical properties of the cellular transepithelial pathway inNecturus gallbladder

I. Circuit analysis and steady-state effects of mucosal solution ionic substitutions
  • Luis Reuss
  • Arthur L. Finn
Article

Summary

Microelectrode techniques were employed to measure the electrical resistance of the cell membranes and the shunt pathway, and the equivalent electromotive forces (EMF's) at both cell borders inNecturus gallbladder epithelium. The cell is, on the average, 57 mV negative to the mucosal solution and 59 mV negative to the serosal solution. The transepithelial potential (Vms) ranges from 0.5 to 5 mV, serosal solution positive. Assuming that the shunt EMF (Vs) is zero with standard Ringer's bathing both sides of the tissue, both cell membrane EMF's are oriented with the negative pole toward the cell interior and are 39.9±3.6 mV (apical,Va), and 69.4±1.8 mV (basal-lateral,Vb). The values of the resistances of the cell membranes and the shunt are similar to those previously reported by others: apical (Ra), 3350±390 Ω cm2, basal-lateral (Rb) 2750±320 Ω cm2, shunt (Rs), 480±50 Ω cm2. Ionic substitutions on the mucosal side produce changes in both EMF and resistance of the apical membrane and the shunt pathway. Increasing K concentration to 112mm reversesVa and greatly reducesRa. Complete Na replacement with an inert nonpermeant cation slightly increasesVa andRa. These results indicate that across the apical membranePK>PNa. Analogous measurements ofVs indicate cation permselectivity, withPK>PNa>PcholinePTEAPmethylglucamine. In general, changes inVs are very similar to the changes inVms, indicating that the latter measurements yield adequate information on the properties of the shunt. The fact thatPNa>PCl across the shunt rules out the possibility thatVms is generated by a NaCl concentration gradient across the limiting junction.

Keywords

Apical Membrane Negative Pole Electromotive Force Produce Change Cell Border 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc 1975

Authors and Affiliations

  • Luis Reuss
    • 1
  • Arthur L. Finn
    • 1
  1. 1.Department of MedicineUniversity of North Carolina School of MedicineChapel Hill

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