Summary
Amino acids enter rabbit jejunal brush border membrane vesicles via three major transport systems: (1) simple passive diffusion; (2) Na-independent carriers; and (3) Na-dependent carriers. The passive permeability sequence of amino acids is very similar to that observed in other studies involving natural and artificial membranes. Based on uptake kinetics and cross-inhibition profiles, at least two Na-independent and three Na-dependent carrier-mediated pathways exist. One Na-independent pathway, similar to the classical L system, favors neutral amino acids, while the other pathway favors dibasic amino acids such as lysine. One Na-dependent pathway primarily serves neutrall-amino acids including 2-amino-2-norbornanecarboxylic acid hemihydrate (BCH), but not β-alanine or α-methylaminoisobutyric acid (MeAIB). Another Na-dependent route favors phenylalanine and methionine, while the third pathway is selective for imino acids and MeAIB. Li is unable to substitute for Na in these systems. Cross-inhibition profiles indicated that none of the Na-dependent systems conform to classical A or ACS paradigms. Other notable features of jejunal brush border vesicles include (1) no β-alanine carrier, and (2) no major proline/glycine interactions.
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References
Akedo, H., Christensen, H.N. 1962. Nature of insulin action on amino acid uptake by the isolated diaphragm.J. Biol. Chem. 237:118–122
Christensen, H.N. 1964. Relations in the transport of β-alanine and the α-amino acids in the Ehrlich cell.J. Biol. Chem. 239:3584–3589
Christensen, H.N. 1969. Some special kinetic problems of transport.Adv. Enzymol. 32:1–20
Christensen, H.N. 1975. Biological Transport. W.A. Benjamin, Inc., Reading, Mass.
Christensen, H.N. 1979. Exploiting amino acid structure to learn about membrane transport.Adv. Enzymol. 49:41–101
Christensen, H.N., Antonioli, J.A. 1969. Cationic amino acid transport in the rabbit reticulocyte. Na+-dependent inhibition of Na+-independent transport.J. Biol. Chem. 244:1497–1504
Christensen, H.N., Handlogten, M.E., Lam, I., Tager, H.S., Zand, R. 1969. A bicyclic amino acid to improve discriminations among transport systems.J. Biol. Chem. 244:1510–1520
Christensen, H.N., Liang, M. 1966. Transport of diamino acids into the Ehrlich cell.J. Biol. Chem. 214:5542–5551
Christensen, H.N., Liang, M., Archer, E.G. 1967. A distinct Na+-requiring transport system for alanine, serine, cystine, and similar amino acids.J. Biol. Chem. 242:5237–5246
Christensen, H.N., Oxender, D.L., Liang, M., Vatz, K.A. 1965. The use of N-methylation to direct the route of mediated transport of amino acids.J. Biol. Chem. 240:3609–3616
Crane, R.K. 1960. Intestinal absorption of sugars.Physiol. Rev. 40:789–825
Crane, R.K. 1962. Hypothesis of mechanisms of intestinal active transport of sugars.Fed. Proc. 21:891–895
Diamond, J.M., Wright, E.M. 1969. Biological membranes: The physical basis of ion and nonelectrolyte selectivity.Annu. Rev. Physiol. 31:581–646
Hagihira, H., Wilson, T.H., Lin, E.C.C. 1962. Intestinal transport of certain N-substituted amino acids.Am. J. Physiol. 203:637–640
Hajjar, J.J., Curran, P.F. 1970. Characteristics of the amino acid transport system in the mucosal border of rabbit ileum.J. Gen. Physiol. 56:637–691
Jacquez, J.A., Sherman, J.H., Terris, J. 1970. Temperature dependence of amino acid transport in Ehrlich ascites cells: With results which bear on the A-L distinction.Biochim. Biophys. Acta 203:150–166
Kessler, M., Acuto, O., Storelli, C., Murer, H., Muller, M., Semenza, G. 1978. A modified procedure for the rapid preparation of efficiently transporting vesicles from small intestinal brush border membranes. Their use in investigating some properties ofd-glucose and choline transport systems.Biochim. Biophys. Acta 506:136–154
Kilberg, M.S., Christensen, H.N., Handlogten, M.E. 1979. Cysteine as a system-specific substrate for transport system ASC in rat hepatocytes.Biochem. Biophys. Res. Commun. 88:744–751
Klein, R.A., Moore, M.J., Smith, M.W. 1971. Selective diffusion of neutral amino acids across lipid bilayers.Biochim. Biophys. Acta 233:420–433
Lever, J.E. 1980. The use of membrane vesicles in transport studies.CRC Crit. Rev. Biochem. 7:187–246
Lin, E.C.C., Hagihira, H., Wilson, T.H. 1962. Specificity of the transport system for neutral amino acids in the hamster intestine.Am. J. Physiol. 202:919–925
McClellan, W.M., Schafer, J.A. 1973. Transport of amino-acid analog, 2-aminobicyclo(2,2)-heptane-2-carboxylic acid, by Ehrlich ascites tumor cells.Biochim. Biophys. Acta 311:462–475
Mircheff, A.K., Kippen, I., Hirayama, B., Wright, E.M. 1982. Delineation of sodium-stimulated amino acid transport pathways in rabbit kidney brush border vesicles.J. Membrane Biol. 64:113–122
Mircheff, A.K., Os, C.H. van, Wright, E.M. 1980. Pathways for alanine transport in intestinal basal membrane vesicles.J. Membrane Biol. 52:83–92
Mircheff, A.K., Wright, E.M. 1976. Analytical isolation of plasma membranes of intestinal epithelial cells. Identification of Na, K-ATPase rich membranes and the distribution of enzyme activities.J. Membrane Biol. 28:309–333
Mitchell, M.A., Levin, R.J. 1981. Amino acid absorption in jejunum and ileum in vivo — a kinetic comparison of function on surface area and regional bases.Experientia 37:265–266
Munck, B.G. 1966. Amino acid transport by the small intestine of the rat. The existance and specificity of the transport mechanisms of imino acids and its relation to the transport of glycine.Biochim. Biophys. Acta 120:97–103
Munck, B.G. 1980. Lysine transport across the small intestine. Stimulating and inhibitory effects of neutral amino acids.J. Membrane Biol. 53:45–53
Munck, B.G. 1981. Intestinal absorption of amino acids.In: Physiology of the Gastrointestinal Tract. L.R. Johnson, J. Christensen, M.L. Grossman, E.D. Jacobson, and S.G. Schultz, editors. pp. 1097–1122, Raven Press, New York
Munck, B.G., Schultz, S.G. 1969. Lysine transport across isolated rabbit ileum.J. Gen. Physiol. 53:157–182
Newey, H., Smyth, D.H. 1964. The transfer system for neutral amino acids in the rat small intestine.J. Physiol. (London) 170:328–343
Oxender, D.L., Christensen, H.N. 1963. Distinct mediating systems for the transport of neutral amino acids by the Ehrlich cell.J. Biol. Chem. 238:3686–3699
Paterson, J.Y.F., Sepulveda, F.V., Smith, M.W. 1979. Two-carrier influx of neutral amino acids into rabbit ileal mucosa.J. Physiol. (London) 292:339–350
Paterson, J.Y.F., Smith, M.W., Sepulveda, F.V. 1980. Animal variation in alanine uptake by rabbit ileal mucosa.Biochim. Biophys. Acta 598:184–188
Preston, R.L., Schaffer, J.F., Curran, P.F. 1974. Structure-affinity relationships of substrates for the neutral amino acid transport system in rabbit ileum.J. Gen. Physiol. 64:443–467
Schmitz, J., Preiser, H., Maestracci, D., Ghosh, B.K., Cerda, J.J., Crane, R.K. 1973. Purification of the human intestinal brush border membrane.Biochim. Biophys. Acta 323:98–112
Schultz, S.G., Curran, P.F. 1970. Coupled transport of sodium and organic solutes.Physiol. Rev. 50:637–718
Schultz, S.G., Markscheid-Kaspi, L. 1971. Competitive interactions betweenl-alanine andl-phenylalanine in rabbit ileum.Biochim. Biophys. Acta 241:857–860
Segal, I.H. 1975. Enzyme Kinetics. John Wiley and Sons, New York
Sepulveda, F.V., Smith, M.W. 1978. Discrimination between different entry mechanisms for neutral amino acids in rabbit ileal mucosa.J. Physiol. (London) 282:73–90
Silbernagl, S., Foulkes, E.C., Deetjen, P. 1975. Renal handling of amino acids.Rev. Physiol. Biochem. Pharmacol. 74:105–167
Silverman, M., Turner, R.J. 1980. Renal proximal tubule.In: Biomembranes. L.A. Manson, editor. Vol. 10, pp. 1–50. Plenum Press, New York
Smith, M.W., Sepulveda, F.V. 1979. Sodium dependence of neutral amino acid uptake into rabbit ileum.Biochim. Biophys. Acta 555:374–378
Spears, G., Sneyd, J.G.T., Loten, E.G. 1971. A method for deriving kinetic constants for two enzymes acting on the same substrate.Biochem. J. 125:1149–1151
Spencer, R.P., Bow, T.M., Markulis, M.A. 1962. Amino group requirements for in vitro intestinal transport of amino acids.Am. J. Physiol. 202:171–173
Stevens, B.R. 1980. The role of sulfhydryl groups in alanine transport by lyophilized brush border membrane vesicles. Ph. D. Dissertation. Illinois State University, Normal
Stevens, B.R., Wright, S.H., Hirayama, B., Ross, H.J., Gunther, R., Nord, E., Kippen, I., Wright, E.M. 1982. Organic and inorganic solute transport in renal and intestinal membrane vesicles preserved in liquid nitrogen.J. Membrane Biochem. (in press)
Tager, H.S., Christensen, H.N. 1971. Transport of the four isomers of 2-aminonorborane-2-carboxylic acid inEscherichia coli.J. Biol. Chem. 246:7572–7580
Ullrich, K.J., Runrich, G., Kloss, S. 1974. Sodium-dependence of the amino acid transport in the proximal convolution of the rat kidney.Pfluegers. Arch. 351:49–60
Wilson, P.D., Wheeler, K.P. 1973. Permeability of phospholipid vesicles to amino acids.Biochem. Soc. Trans. 1:369–372
Young, J.D., Ellory, J.C. 1977. Red. cell amino acid transport.In: Membrane Transport in Red Cells. J.C. Ellory and V.L. Lew, editors, pp. 301–325. Academic Press, London
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Stevens, B.R., Ross, H.J. & Wright, E.M. Multiple transport pathways for neutral amino acids in rabbit jejunal brush border vesicles. J. Membrain Biol. 66, 213–225 (1982). https://doi.org/10.1007/BF01868496
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DOI: https://doi.org/10.1007/BF01868496