The Journal of Membrane Biology

, Volume 116, Issue 3, pp 261–272 | Cite as

Constant turnover of arachidonic acid and inhibition of a potassium current inAplysia giant neurons

  • Robert O. Carlson
  • Irwin B. Levitan


Steady-state currents at hyperpolarized membrane potentials were studied in the homologous giant neurons, LP1 and R2, ofAplysia using two-electrode voltage clamp. Nearly half of the steady-state current at voltages more hyperpolarized than −70 mV had characteristics similar to the inwardly rectifying potassium current (IR) described previously inAplysia neurons. The pharmacological agents 4-bromophenacylbromide, indomethacin, and the phorbol ester, 12-O-tetradecanoyl-phorbol-13-acetate were found to modulateIR.IR was stimulated with BPB and indomethacin and inhibited with TPA. These agents alteredIR by a mechanism independent ofcAMP, which can also modulateIR. The effects of these modulators are consistent with their actions on arachidonic acid (AA) metabolism inAplysia nervous system, suggesting AA may constitutively inhibitIR. When ganglia were perfused for 12 hr with medium containing BSA to absorb extracellular fatty acids,IR was increased nearly twofold. This increase was partially inhibited by addition of AA to the perfusion medium, and completely inhibited by pretreatment of ganglia with BPB. Although no direct effect of shortterm exposure to exogenous AA was observed, long term exposure to exogenous AA and several other unsaturated fatty acids was accompanied by a decrease inIR.

Key Words

arachidonic acid eicosanoids Aplysia inward rectifier phorbol ester potassium current protein kinase C 


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Copyright information

© Springer-Verlag New York Inc. 1990

Authors and Affiliations

  • Robert O. Carlson
    • 1
  • Irwin B. Levitan
    • 1
  1. 1.Graduate Department of BiochemistryBrandeis UniversityWaltham

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