The Journal of Membrane Biology

, Volume 36, Issue 1, pp 55–63 | Cite as

Contribution of mucosal chloride to chloride in toad bladder epithelial cells

  • Anthony D. C. Macknight
Article

Summary

Epithelial cells were scraped from the bladders of toads of the speciesBufo marinus obtained from the Dominican Republic. These epithelial cells exchanged their chloride virtually completely with36Cl in the medium within 60 min. Of this chloride, about 93% came from the serosal medium. The approximately 20 mmole/kg dry wt of chloride which equilibrates with36Cl in the mucosal medium was still present when choline replaced sodium in the medium in the presence of amiloride (10−4 M) and was almost all readily removed by rapid washing of the mucosal surface immediately prior to analysis. These observations suggest that little chloride of mucosal origin is truly intracellular. This conclusion is supported by the fact that after vasopressin the increased cellular chloride was not of mucosal origin.

Keywords

Sodium Chloride Epithelial Cell Choline Human Physiology 

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References

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Copyright information

© Springer-Verlag New York Inc. 1977

Authors and Affiliations

  • Anthony D. C. Macknight
    • 1
  1. 1.Department of PhysiologyUniversity of Otago Medical SchoolDunedinNew Zealand

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