The Journal of Membrane Biology

, Volume 36, Issue 1, pp 55–63 | Cite as

Contribution of mucosal chloride to chloride in toad bladder epithelial cells

  • Anthony D. C. Macknight


Epithelial cells were scraped from the bladders of toads of the speciesBufo marinus obtained from the Dominican Republic. These epithelial cells exchanged their chloride virtually completely with36Cl in the medium within 60 min. Of this chloride, about 93% came from the serosal medium. The approximately 20 mmole/kg dry wt of chloride which equilibrates with36Cl in the mucosal medium was still present when choline replaced sodium in the medium in the presence of amiloride (10−4 M) and was almost all readily removed by rapid washing of the mucosal surface immediately prior to analysis. These observations suggest that little chloride of mucosal origin is truly intracellular. This conclusion is supported by the fact that after vasopressin the increased cellular chloride was not of mucosal origin.


Sodium Chloride Epithelial Cell Choline Human Physiology 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Alvarado, R.H., Dietz, T.H., Mullen, T.L. 1975. Chloride transport across isolated skin ofRana pipiens.Am. J. Physiol. 229:869PubMedGoogle Scholar
  2. Davies, H.E.F., Martin, D.G., Sharp, G.W.G. 1968. Differences in the physiological characteristics of bladders of toads from different geographical sources.Biochim. Biophys. Acta 150:315PubMedGoogle Scholar
  3. Finn, A.L., Handler, J.S., Orloff, J. 1967. Active chloride transport in the isolated toad bladder.Am. J. Physiol. 213:179PubMedGoogle Scholar
  4. Finn, A.L., Nellans, H. 1972. The kinetics and distribution of potassium in the toad bladder.J. Membrane Biol. 8:189Google Scholar
  5. Gatzy, J.T. 1971. The effect of potassium-sparing diuretics on ion transport across the excised toad bladder.J. Pharmacol. Exp. Ther. 176:580PubMedGoogle Scholar
  6. Kristensen, P. 1972. Chloride transport across isolated frog skin.Acta Physiol. Scand. 84:338PubMedGoogle Scholar
  7. Leaf, A. 1965. Transepithelial transport and its hormonal control in toad bladder.Ergeb. Physiol. Biol. Chem. Exp. Pharmakol. 56:216Google Scholar
  8. Macknight, A.D.C., DiBona, D.R., Leaf, A., Civan, M.M. 1971a. Measurement of the composition of epithelial cells from the toad urinary bladder.J. Membrane Biol. 6:108Google Scholar
  9. Macknight, A.D.C., Leaf, A., Civan, M.M. 1971b. Effects of vasopressin on the water and ionic composition of toad bladder epithelial cells.J. Membrane Biol. 6:127Google Scholar
  10. Macknight, A.D.C., Civan, M.M., Leaf, A. 1975. The sodium transport pool in toad urinary bladder epithelial cells.J. Membrane Biol. 20:365Google Scholar
  11. McIver, D.J.L., Macknight, A.D.C. 1974. Extracellular space in some isolated tissues.J. Physiol. (London) 239:31Google Scholar
  12. Robinson, B.A., Macknight, A.D.C. 1976. Relationships between serosal medium potassium concentration and sodium transport in toad urinary bladder. III. Exchangeability of epithelial cellular potassium.J. Membrane Biol. 26:269Google Scholar
  13. Schneider, W. 1975. Chloride transport in isolated skin ofRana esculenta.Pfluegers Arch. 355:107Google Scholar
  14. Watlington, C.O., Jessee, F. 1975. Net Cl flux in short-circuited skin ofRana Pipiens: Ouabain sensitivity and Na++K+ dependence.Biochim. Biophys. Acta 382:204PubMedGoogle Scholar

Copyright information

© Springer-Verlag New York Inc. 1977

Authors and Affiliations

  • Anthony D. C. Macknight
    • 1
  1. 1.Department of PhysiologyUniversity of Otago Medical SchoolDunedinNew Zealand

Personalised recommendations