The Journal of Membrane Biology

, Volume 9, Issue 1, pp 57–70 | Cite as

Permeability to water, dimension of surface, and structural changes during swelling in rat liver mitochondria

  • S. Massari
  • L. Frigeri
  • G. F. Azzone
Article

Summary

Rates and amounts of water translocation across the mitochondrial membrane have been studied with a photometric technique. The process of water translocation can be described in terms of the diffusion equations, and the mitochondria behave as spherical bodies between 15 and 110 mosm. A permeability coefficient to water of 5.3×10−3cm sec−1 has been calculated. The mitochondrial surface is about 1m2/g protein during incubation in 0.10m KCl, and increases to 30 m2/g protein during incubation in 0.005m KCl.

The osmotic shrinkage of hypotonically swollen mitochondria has also been studied. Complete reversibility of hypotonic swelling occurs only after incubation of mitochondria in media below 60 to 90 mosm. The appearance of the reversibility is phenomenologically correlated with the rupture of the outer mitochondrial membrane., Below 30 mosm there is a change of the absorbance properties of the membrane. The change correlates with the complete unfolding of the cristae and is attributed to ultrastructural reorganization of the membrane following mechanical stretching.

Keywords

Structural Change Human Physiology Mitochondrial Membrane Permeability Coefficient Diffusion Equation 

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References

  1. Bangham, A. D., De Gier, J., Greville, G. D. 1967. Osmotic properties and water permeability of phospholipid liquid crystals.Chem. Phys. Lipids 1:225.Google Scholar
  2. Bentzel, C. J., Solomon, A. K. 1967. Osmotic properties of mitochondria.J. Gen. Physiol. 50:1947.Google Scholar
  3. Cass, A., Finkelstein, A. 1967. Water permeability of thin lipid membranes.J. Gen. Physiol. 50:1765.PubMedGoogle Scholar
  4. Estabrook, R. W., Holowinsky, A. 1961. Studies on the content and organization of the respiratory enzymes of mitochondria.J. Biophys. Biochem. Cytol. 9:19.PubMedGoogle Scholar
  5. Kaplan, A. I., Greenawalt, J. W. 1966. Biochemical and ultrastructural properties of osmotically lysed rat-liver mitochondria.J. Cell. Biol. 31:455.PubMedGoogle Scholar
  6. Lehninger, A. L. 1965. The Mitochondrion. W. A. Benjamin, New York. p. 30.Google Scholar
  7. Mitchell, P. 1966. Metabolic flow in the mitochondrial multiphase system: An appraisal of the chemi-osmotic theory of oxidative phosphorylation.In: Regulation of Metabolic Processes in Mitochondria. S. M. Tager, S. Papa, E. Quagliariello, and E. C. Slater, editors. Vol. 7, p. 65. Elsevier BBA Library, Amsterdam.Google Scholar
  8. Müller, P., Rudin, D. O., 1969. Translocators in bimolecular lipid membranes: Their role in dissipative bioenergetic transduction.Curr. Top. Bioenerget. 3:157.Google Scholar
  9. Rossi, E., Azzone, G. F. 1969. Ion transport in liver mitochondria. Energy barrier and stoicheometry of aerobic K+ translocation.Europ. J. Biochem. 7:418.PubMedGoogle Scholar
  10. Stoner, C. D., Sirak, H. D. 1969. Osmotically induced alterations in volume and ultrastructure of mitochondria isolated from rat liver and bovine heart.J. Cell. Biol. 43:521.PubMedGoogle Scholar
  11. Tedeschi, H. 1959. The structure of mitochondrial membrane: Inferences from permeability properties.J. Biophys. Biochem. Cytol. 6:241.PubMedGoogle Scholar
  12. Tedeschi, H. 1961. Osmotic reversal of mitochondrial swelling.Biochim. Biophys. Acta 46:159.PubMedGoogle Scholar
  13. Tedeschi, H., Harris, D. L. 1955. The osmotic behaviour and permeability to ionelectrolytes of mitochondria.Arch. Biochem. Biophys. 58:52.PubMedGoogle Scholar
  14. Tedeschi, H., Harris, D. L. 1958. Some observations on the photometric estimation of mitochondrial volume.Biochim. Biophys. Acta 28:392.PubMedGoogle Scholar
  15. Yoshida, K., Sato, S. 1968. Swelling and contraction of isolated plant mitochondria. I. Passive swelling in sugar and electrolyte solutions.J. Faculty Sci., Univ. Tokyo 10:49.Google Scholar
  16. Whittaker, V. P. 1966. The ultrastructure of mitochondria.In: Regulation of Metabolic Processes in Mitochondria. J. M. Tager, S. Papa, E. Quagliariello, and E. C. Slater, editors. Vol. 7, p. 1. Elsevier BBA Library, Amsterdam.Google Scholar

Copyright information

© Springer-Verlag New York Inc. 1972

Authors and Affiliations

  • S. Massari
    • 1
  • L. Frigeri
  • G. F. Azzone
    • 1
  1. 1.C.N.R. Unit for the Study of Physiology of Mitochondria, Institute of General PathologyUniversity of PadovaItaly

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