As background to a detailed analysis of the cation permeation mechanism in rabbit gallbladder epithelium, this paper considers several general problems in interpretation. With regard to liquid junction potentials, the common practice of using saturated KCl bridges was insufficiently accurate for the present purposes because the resulting junctions are time-dependent and poorly understood theoretically. Time-independent and well-defined junction potentials were obtained by arranging all junctions to be of the biionic or single-salt dilution types. The magnitudes of these junction potentials were estimated in three different ways, with good agreement. Recording arrangements using either agar bridges or else Ag/AgCl electrodes also yielded good agreement after appropriate corrections for junction potentials and electrode potentials. The effects of nonelectrolytes on electrode standard potentials were measured. Two experiments were devised to determine whether transepithelially measured electrical properties of the gallbladder refer to a single membrane or to two membranes in series: the potential difference change resulting from a mucosal concentration change was measured as a function of the serosal concentration, and intracellular concentrations were altered by increasing bathing solution osmolalities with an impermeant nonelectrolyte. Both types of experiment indicated that transepithelial measurements are dominated by a single membrane. Small corrections were applied to measured potential differences to take account of unstirred-layer effects with permeant salts.
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Barry, P. H., Diamond, J. M., Wright, E. M. 1970. The mechanism of cation permeation in rabbit gallbladder: Dilution potentials and biionic potentials. (in preparation).
Brightman, M. W., Reese, T. S. 1969. Junction between intimately apposed cell membranes in the vertebrate brain.J. Cell Biol. 40:648.
Caldwell, P. C. 1968. Liquid junction potentials and their effect on potential measurements in biological systems.Int. Rev. Cytol. 24:345.
Diamond, J. M. 1962a. The reabsorptive function of the gall-bladder.J. Physiol. 161:442.
— 1962b. The mechanism of solute transport by the gall-bladder.J. Physiol. 161:474.
— 1964. Transport of salt and water in rabbit and guinea pig gall-bladder.J. Gen Physiol. 48:1.
— 1966. A rapid method for determining voltage-concentration relations across membranes.J. Physiol. 183:83.
— 1968. Transport mechanisms in the gall-bladder.In: Handbook of Physiology, vol. 5. p. 2451. Williams and Wilkins, Baltimore.
—, Harrison, S. C. 1966. The effect of membrane fixed charges on diffusion potentials and streaming potentials.J. Physiol. 183:37.
Eisenman, G. 1965. The electrochemistry of cation-sensitive glass electrodes.In: Advances in Analytical Chemistry and Instrumentation, vol. 4. C. N. Reilley, editor. Interscience Publishers, New York.
Garrels, R. M. 1967. Ion-sensitive electrodes and individual ion activity coefficients.In: Glass Electrodes for Hydrogen and Other Cations. G. Eisenman, editor. p. 344. Marcel Dekker, New York.
Kelly, F. J., Robinson, R. A., Stokes, R. H. 1961. The thermodynamics of the ternary system mannitol-sodium chloride — water at 25o from solubility and vapour pressure measurements.J. Phys. Chem. 65:1958.
MacInnes, D. A. 1961. The Principles of Electrochemistry. Dover Publications, New York.
Robinson, R. A., Stokes, R. H. 1962. Activity coefficients of mannitol and potassium chloride in mixed aqueous solutions at 25o.J. Phys. Chem. 66:506.
—— 1965. Electrolyte Solutions. Butterworths, London.
Sandblom, J. P., Eisenman, G. 1967. Membrane potentials at zero current. The significance of a constant ionic permeability ratio.Biophys. J. 7:217.
Teorell, T. 1953. Transport processes and electrical phenomena in ionic membranes.Prog. Biophys. Biophys. Chem. 3:305.
Tormey, J. McD., Diamond, J. M. 1967. The ultrastructural route of fluid transport in rabbit gall-bladder.J. Gen. Physiol. 50:2031.
Ussing, H. H., Windhager, E. E. 1964. Nature of shunt path and active sodium transport path through frog skin epithelium.Acta Physiol. Scand. 61:484.
Windhager, E. E., Boulpaep, E. L., Giebisch, G. 1967. Electrophysiological studies on single nephrons.In: Proc. 3rd Int. Congr. Nephrol. (Washington, D.C., 1966), vol. 1, p. 35. Karger, Basel-New York.
Wright, E. M., Barry, P. H., Diamond, J. M. 1970. The mechanism of cation permeation in rabbit gall-bladder: Conductances, the concentration dependence of anion-cation discrimination, and the calcium competition effect (in preparation).
—, Diamond, J. M. 1968. Effects of pH and polyvalent cations on the selective permeability of gall-bladder epithelium to monovalent ions.Biochim. Biophys. Acta 163:57.
—— 1969. An electrical method of measuring non-electrolyte permeability.Proc. Roy. Soc. (London) B. 172:203.
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Barry, P.H., Diamond, J.M. Junction potentials, electrode standard potentials, and other problems in interpreting electrical properties of membranes. J. Membrain Biol. 3, 93–122 (1970). https://doi.org/10.1007/BF01868010
- Junction Potential
- Single Membrane
- Liquid Junction Potential
- Electrode Standard Potential
- Gallbladder Epithelium