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The Journal of Membrane Biology

, Volume 7, Issue 1, pp 313–324 | Cite as

On the impermeability of the outer mitochondrial membrane to cytochromec

II. Studies on isolated membrane fragments
  • Lech Wojtczak
  • G. L. Sottocasa
Article

Summary

Outer mitochondrial membranes isolated by the swelling-shrinkage sonication procedure of Sottocasaet al. [19, 20] forms small sealed vesicles. If cytochromec is present during the procedure it is trapped inside these vesicles and can not be washed out nor is accessible to external enzymes, e.g., cytochrome oxidase (EC 1.9.3.1) or succinate-cytochromec reductase present as contamination by the inner membrane, but is fully accessible to rotenone-insensitive NADH-cytochromec reductase of the outer membrane. This indicates the impermeability of the outer mitochondrial membrane to cytochromec.

A modification of the original procedure for the separation of the outer mitochondrial membrane is described.

Keywords

Human Physiology Mitochondrial Membrane Outer Membrane Cytochrome Oxidase Outer Mitochondrial Membrane 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc. 1972

Authors and Affiliations

  • Lech Wojtczak
    • 1
  • G. L. Sottocasa
    • 1
  1. 1.Istituto di Chimica BiologicaUniversity of TriesteTriesteItaly

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