The Journal of Membrane Biology

, Volume 7, Issue 1, pp 88–110 | Cite as

Model approaches for evaluation of cell coupling in monolayers

  • J. Siegenbeek van Heukelom
  • J. J. Denier van der Gon
  • F. J. A. Prop
Article

Summary

The electrical coupling of epithelial cells of the intestine of chick embryo cultured in monolayers was studied. This coupling can be evaluated by regarding the monolayer as a honeycomb structure of cells and the cells as equipotential spaces, as long as the cells are small (diameter <25μm). With help of this discrete model it was found that for the non-junctional membrane the membrane resistance isϱ m =250−2,000 Ω cm2, and for the junctional membraneϱ m =5−50 Ω cm2. In addition to this discrete model, a continuous model was also considered and good agreement between the two descriptions was found. With the aid of the continuous model, a value for the non-junctional membrane capacitance (C m ) was obtained: 5–50 μF/cm2. The electrical values are not corrected for membrane folding, microvilli and the like. Tentative corrections based on electron microscopy suggest: 1,000 <ϱ m < 10,000 Ω cm2, 10 <ϱ m < 100 Ω cm2, 1<C m < 10 μF/cm2

Keywords

Electron Microscopy Epithelial Cell Human Physiology Continuous Model Chick Embryo 

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Copyright information

© Springer-Verlag New York Inc. 1972

Authors and Affiliations

  • J. Siegenbeek van Heukelom
    • 1
    • 2
  • J. J. Denier van der Gon
    • 1
    • 2
  • F. J. A. Prop
    • 1
    • 2
  1. 1.Medical Physics Department, Physical LaboratoryState University of UtrechtUtrechtThe Netherlands
  2. 2.Pathological-Anatomy LaboratoryUniversity of AmsterdamAmsterdamThe Netherlands

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