The Journal of Membrane Biology

, Volume 10, Issue 1, pp 45–66 | Cite as

Kinetic analysis of calcium movements in cell culture

V. Intracellular calcium distribution in kidney cells
  • André B. Borle


The distribution of intracellular calcium was determined in isolated kidney cells by kinetic analyses of45Ca fluxes. Isotopic desaturation curves reveal an intracellular calcium compartment with a very slow time constant. The size of this calcium compartment is markedly increased by raising the extracellular calcium, by increasing the extracellular phosphate and may contain up to 99% of the intracellular exchangeable calcium. Accumulation of calcium in this pool is completely abolished by two specific inhibitors of mitochondrial calcium uptake, Antimycin A and Warfarin®. These results suggest that this compartment represents a pool of calcium in the cell mitochondria. The sudden removal of phosphate from the medium immediately stimulates calcium efflux from the cell. Conversely, an increase in medium phosphate immediately inhibits calcium efflux. Both effects are rapidly reversible. Finally, calcium efflux from the cells is stimulated after the cells are exposed to low temperature suggesting that calcium transport out of the cell may be regulated by the cytoplasmic calcium activity. These experiments are consistent with the view that mitochondria play an important role in the control and regulation of cytoplasmic calcium activity and of calcium transport.


Warfarin Intracellular Calcium Kinetic Analysis Extracellular Calcium Calcium Transport 
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  1. Ahren, K., Hjalmarson, A., Isaksson, O. 1971. Inotropic and metabolic effects of dibutyryl cyclic adenosine 3′,5′-monophosphate in the perfused rat heart.Acta Physiol. Scand. 82:79.PubMedGoogle Scholar
  2. Blaustein, M. P., Hodgkin, A. L. 1969. The effect of cyanide on the efflux of calcium from squid axon.J. Physiol. (London) 200:497.Google Scholar
  3. Borle, A. B. 1967. Membrane transfer of calcium.Clin. Orthop. Related Res. 52:267.Google Scholar
  4. Borle, A. B. 1969a. Kinetic analyses of calcium movements in Hela cell cultures. I. Calcium influx.J. Gen. Physiol. 53:43.PubMedGoogle Scholar
  5. Borle, A. B. 1969b. Kinetic analyses of calcium movements in Hela cell cultures. II. Calcium efflux.J. Gen. Physiol. 53:57.PubMedGoogle Scholar
  6. Borle, A. B. 1970. Kinetic analyses of calcium movements in cell cultures. III. Effects of calcium and parathyroid hormones in kidney cells.J. Gen. Physiol. 55:163.PubMedGoogle Scholar
  7. Borle, A. B. 1971a. Regulation of calcium transport in intestinal cells by calciferol (D3).Endocrinology 88:A-155.Google Scholar
  8. Borle, A. B. 1971b. Le turn-over du calcium dans l'organisme et les principaux points d'impact hormonaux.In: Les Hormones et le Calcium. H. P. Klotz, editor. p. 5. Expansion Scientifique Francaise. Paris.Google Scholar
  9. Borle, A. B. 1972. Parathyroid hormone and cell calcium.In: Calcium, Parathyroid Hormone and the Calcitonins R. V. Talmage and P. Munson, editors. p. 484. Excerpta Medica, Amsterdam.Google Scholar
  10. Drahota, Z., Carafoli, E., Rossi, C. S., Gamble, R. L., Lehninger, A. L. 1965. The steady maintenance of accumulated Ca++ in rat liver mitochondria.J. Biol. Chem. 240:2713.Google Scholar
  11. Hodgkin, A. L., Keynes, R. D. 1957. Movement of labeled calcium in giant axons.J. Physiol. (London) 138:253.Google Scholar
  12. Horn, R. S., Fyhn, A., Haugaard, N. 1970. Mitochondrial calcium uptake in the perfused contracting rat heart and the influence of epinephrine on calcium exchange.Biochim. Biophys. Acta 226:459.Google Scholar
  13. Huxley, A. F. 1960. A. K. Solomon Compartmental Methods of Kinetic Analyses (Appendix).In: Mineral Metabolism. Vol. 1, Pt. A., p. 119. C. L. Comar and F. Bronner, editors. Academic Press Inc., New York.Google Scholar
  14. Isaacson, A., Sandow, A. 1967. Quinine and caffeine effects on45Ca movements in frog sartorius muscle.J. Gen. Physiol. 50:2109.PubMedGoogle Scholar
  15. Kimberg, D. V., Goldstein, S. A. 1967. Binding of calcium by liver mitochondria: An effect of steroid hormones in vitamin D depleted and parathyroidectomized rats.Endocrinology 80:89.PubMedGoogle Scholar
  16. Langer, G. A. 1964. Kinetic studies of calcium distribution in ventricular muscle of the dog.Circulation Res. 15:393.PubMedGoogle Scholar
  17. Lehninger, A. L. 1964. The Mitochondrion. W. A. Benjamin, New York. p. 263.Google Scholar
  18. Lehninger, A. L. 1970. Mitochondria and calcium transport.Biochem. J. 119:129.PubMedGoogle Scholar
  19. Martin, J. H., Matthews, J. L. 1969. Mitochondrial granules in chondrocytes osteoblasts and osteocytes; an ultrastructural and microincineration study.Clin. Orthop. Related Res. 68:273.Google Scholar
  20. Mela, L., Chance, B. 1968. Spectrophotometric measurements of the kinetics of Ca2+ and Mn2+ accumulation in mitochondria.Biochemistry 7:4059.PubMedGoogle Scholar
  21. Peachey, L. D. 1964. Electron microscopic observations on the accumulation of divalent cations in intramitochondrial granules.J. Cell Biol. 20:95.PubMedGoogle Scholar
  22. Rasmussen, H. 1970. Cell communication, calcium ion and cyclic adenosine monophosphate.Science 170:404.PubMedGoogle Scholar
  23. Reynolds, E. S., Thiers, R. E., Vallee, B. L. 1962. Mitochondrial function and metal content in carbon tetrachloride poisoning.J. Biol. Chem. 237:3546.PubMedGoogle Scholar
  24. Rossi, S. R., Carafoli, E., Drahota, Z., Lehninger, A. L. 1966. The stoichiometry and the dynamics of energy-linked accumulation of Ca2+ by mitochondria.In: Regulation of Metabolic Processes in Mitochondria. p. 317. J. M. Tager, S. Papa, E. Quagliariello, and E. C. Slater, editors. Elsevier Publishing Co., Amsterdam.Google Scholar
  25. Sampson, H. W., Matthews, J. L., Martin, J. H., Kunin, A. S. 1970. An electron microscopic localization of calcium in the small intestine of normal rachitic and vitamin D treated rats.Calc. Tiss. Res. 5:305.Google Scholar
  26. Scarpelli, D. G. 1965. Experimental nephrocalcinosis.Lab. Invest. 14:123.PubMedGoogle Scholar
  27. Thiers, R. E., Reynolds, E. S., Vallee, B. L. 1960. The effect of carbon tetrachloride poisoning on subcellular metal distribution in rat liver.J. Biol. Chem. 235:2130.PubMedGoogle Scholar
  28. Winegrad, S., Shanes, A. M. 1962. Calcium flux and contractility in guinea pig atria.J. Gen. Physiol. 45:371.PubMedGoogle Scholar

Copyright information

© Springer-Verlag New York Inc 1972

Authors and Affiliations

  • André B. Borle
    • 1
  1. 1.Department of PhysiologyUniversity of Pittsburgh School of MedicinePittsburgh

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