Research in Experimental Medicine

, Volume 165, Issue 1, pp 49–54 | Cite as

The metabolism and organ distribution of 5,6-trans-25-hydroxycholecalciferol, an analog of vitamin D3 in vitamin D-deficient rats

  • K. Schaefer
  • D. Kraft
  • D. von Herrath
  • H. -G. Grigoleit


Investigations have been performed on the metabolism and organ distribution of 5,6-trans-25(OH)D3, which represents an analog of vitamin D3 being active in uremia. This analog is rapidly metabolized into at least two more metabolites. The main storage organ for this compound seems to be fat, whereas liver and intestinal mucosa showed at 24 hrs the highest concentration of polar metabolites. In contrast to 25(OH)D3 5,6-trans-25 (OH)D3 is much less absorbed from the intestinal tract, which should be considered if this compound is applied for treatment of uremic bone diseases.

Key words

Vitamin D Renal osteodystrophy 5,6-trans-25-hydroxycholecalciferol Organ distribution Metabolism Rats 


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  1. 1.
    Bligh, E. G., Dyer, W. J.: Canad. J. Biochem. Physiol.37, 911 (1959)PubMedGoogle Scholar
  2. 2.
    Coburn, J. W., Norman, A. W.: Clin. Nephrol.1, 273 (1973)PubMedGoogle Scholar
  3. 3.
    DeLuca, H. F.: New Engl. J. Med.284, 554 (1971)PubMedGoogle Scholar
  4. 4.
    Fraser, D. R., Kodicek, E.: Nature (Lond.)228, 764 (1970)Google Scholar
  5. 5.
    Hallick, R. B., DeLuca, H. F.: J. biol. Chem.247, 91 (1972)PubMedGoogle Scholar
  6. 6.
    Herrath, D. von, Kraft, D., Grigoleit, H.-G., Schaefer, K.: Dtsch. med. Wschr.98, 1379 (1973)PubMedGoogle Scholar
  7. 7.
    Herrath, D. von, Schaefer, K., Kraft, D., Grigoleit, H.-G., Koeppe, P.: Klin. Wschr.51, 979 (1973)CrossRefPubMedGoogle Scholar
  8. 8.
    Holick, M. F., Garabedian, M., DeLuca, H. F.: Biochemistry11, 2715 (1972)CrossRefPubMedGoogle Scholar
  9. 9.
    Kleeman, C. R., Better, O. S.: Kidney International4, 73 (1973)PubMedGoogle Scholar
  10. 10.
    Lawson, D. E. M., Bell, P. A., Kodicek, E.: In: Calcified tissue (eds. H. Czitober and J. Eschberger), p. 401. Facta-Publication 1973Google Scholar
  11. 11.
    Mawer, E. B., Backhouse, J.: Biochem. J.112, 255 (1969)PubMedGoogle Scholar
  12. 12.
    Mawer, E. B., Backhouse, J., Taylor, C. M., Lumb, G. A., Stanbury, S. W.: Lancet1973 I, 626Google Scholar
  13. 13.
    Norman, A. W., Midgett, R. J., Myrtle, J. F., Nowicki, H. G.: Biochem. biophys. Res. Commun.42, 1082 (1971)CrossRefPubMedGoogle Scholar
  14. 14.
    Ponchon, G., DeLuca, H. F.: J. Nutr.99, 157 (1969)PubMedGoogle Scholar
  15. 15.
    Schaefer, K., Herrath, D. von, Stratz, R.: Israel J. med. Sci.8, 80 (1972)PubMedGoogle Scholar
  16. 16.
    Schaefer, K., Herrath, D. von, Opitz, A., Koch, H.-U., Stratz, R.: Europ. J. clin. Invest.2, 133 (1972)PubMedGoogle Scholar
  17. 17.
    Schaefer, K., Herrath, D. von, Kraft, D.: Dtsch. med. Wschr.98, 1338 (1973)PubMedGoogle Scholar
  18. 18.
    Stanbury, S. W.: Clin. Endocr. Metab.1, 267 (1972)Google Scholar

Copyright information

© Springer-Verlag 1975

Authors and Affiliations

  • K. Schaefer
    • 1
    • 2
  • D. Kraft
    • 1
  • D. von Herrath
    • 1
  • H. -G. Grigoleit
    • 1
  1. 1.Medizinische Klinik und Poliklinik, Klinikum SteglitzFree University of BerlinGermany
  2. 2.St. Joseph-Krankenhaus I Medizinische Abteilung IINephrologie und DialyseBerlin 42Germany

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