Research in Experimental Medicine

, Volume 189, Issue 1, pp 9–14 | Cite as

Simulated weightlessness and bone metabolism: Decrease of alkaline phosphatase activity in the femoral diaphysis of rats

  • M. Yamaguchi
  • K. Ozaki
  • T. Hoshi
Original Papers


The effect of simulated weightlessness on bone metabolism was investigated in the skeletal unloading for up to 9 days. The skeletal unloading was designed by using the model of hindlimb hang in rats. The food ingestion of rats with the skeletal unloading was not altered in comparison to that of normal rats. Calcium concentration in the serum was not significantly altered by the skeletal unloading for 9 days, while the serum inorganic phosphorus concentration was significantly decreased at 6 and 9 days. Calcium content in the femoral diaphysis was not altered by the skeletal unloading for 9 days. However, the activities of alkaline and acid phosphatases in the femoral diaphysis were markedly decreased by the skeletal unloading. The decrease in bone alkaline phosphatase activity was seen at 2 days with the skeletal unloading. The present results demonstrate that the skeletal unloading with hindlimb hang can induce the disorder of bone metabolism. This model is useful for studying the effects of simulated weightlessness on bone metabolism.

Key words

Weightlessness Skeletal unloading Bone metabolism Alkaline phosphatase Rat femur 


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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • M. Yamaguchi
    • 1
  • K. Ozaki
    • 1
  • T. Hoshi
    • 2
  1. 1.Dept. of Environmental Biochemistry and ToxicologySchool of Pharmaceutical Sciences, University of ShizuokaShizuoka CityJapan
  2. 2.Laboratory of PhysiologySchool of Food and Nutritional Sciences, University of ShizuokaShizuoka CityJapan

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