Research in Experimental Medicine

, Volume 189, Issue 1, pp 9–14 | Cite as

Simulated weightlessness and bone metabolism: Decrease of alkaline phosphatase activity in the femoral diaphysis of rats

  • M. Yamaguchi
  • K. Ozaki
  • T. Hoshi
Original Papers

Summary

The effect of simulated weightlessness on bone metabolism was investigated in the skeletal unloading for up to 9 days. The skeletal unloading was designed by using the model of hindlimb hang in rats. The food ingestion of rats with the skeletal unloading was not altered in comparison to that of normal rats. Calcium concentration in the serum was not significantly altered by the skeletal unloading for 9 days, while the serum inorganic phosphorus concentration was significantly decreased at 6 and 9 days. Calcium content in the femoral diaphysis was not altered by the skeletal unloading for 9 days. However, the activities of alkaline and acid phosphatases in the femoral diaphysis were markedly decreased by the skeletal unloading. The decrease in bone alkaline phosphatase activity was seen at 2 days with the skeletal unloading. The present results demonstrate that the skeletal unloading with hindlimb hang can induce the disorder of bone metabolism. This model is useful for studying the effects of simulated weightlessness on bone metabolism.

Key words

Weightlessness Skeletal unloading Bone metabolism Alkaline phosphatase Rat femur 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Bikle DD, Halloran BP, Cone CM, Globus RK, Morey-Holton E (1987) The effects of simulated weightlessness on bone maturation. Endocrinology 120:678–684PubMedGoogle Scholar
  2. 2.
    Cann CE, Adachi RR (1983) Bone resorption and mineral excretion in rats during spaceflight. Am J Physiol 244:R327-R331PubMedGoogle Scholar
  3. 3.
    Donaldson CL, Hulley SB, Vogel JM, Hattner RS, Bayers JH, McMillan DE (1970) Effect of prolonged bed rest on bone mineral. Metabolism 19:1071–1077PubMedGoogle Scholar
  4. 4.
    Globus RK, Bikle DD, Morey-Holton ER (1984) Effects of simulated weightlessness on bone mineral metabolism. Endocrinology 114:2264–2270PubMedGoogle Scholar
  5. 5.
    Halloran BP, Bikle DD, Wronski TJ, Globus RK, Levans MJ, Morey-Holton ER (1986) The role of 1,25-dihydroxyvitamin D in the inhibition of bone formation induced by skeletal unloading. Endocrinology 118:948–954PubMedGoogle Scholar
  6. 6.
    Heaney RP (1962) Radiocalcium metabolism in tissue osteoporosis in man. Am J Med 33:188–194PubMedGoogle Scholar
  7. 7.
    Leach CS, Rambaut PC (1977) Biochemical responses of the Skylab crewmen: An overview. In: Johnston RS, Dietlein LF (eds) Biomedical results from Skylab. NASA, Washington, DC, pp 204–216Google Scholar
  8. 8.
    Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–273PubMedGoogle Scholar
  9. 9.
    Morey ER, Baylink DJ (1978) Inhibition of bone formation during spaceflight. Science 201:1138–1141PubMedGoogle Scholar
  10. 10.
    Smith MC Jr, Rambaut PC, Vogel JM, Whittle MW (1977) Bone mineral measurement experiment M078. In: Johnston RS, Dietlin LF (eds) Biomedical results from Skylab. NASA, Washington, DC, pp 183–190Google Scholar
  11. 11.
    Taussky HH, Shon E (1953) A microcolorimetric method for the determination of inorganic phosphorus. J Biol Chem 202:675–685PubMedGoogle Scholar
  12. 12.
    Walter K, Schutt C (1965) Acid and alkaline phosphatase in serum. In: Bergmeyer HU (ed) Methods of enzymatic analysis, vols 1–2. Academic Press, New York, pp 856–860Google Scholar
  13. 13.
    Willis JB (1960) Determination of calcium in blood serum by atomic absorption spectroscopy. Nature 186:249–250PubMedGoogle Scholar
  14. 14.
    Wronski TJ, Morey ER (1982) Skeletal abnormalities in rats induced by simulated weightlessness. Metab Bone Dis Relat Res 4:69–75PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • M. Yamaguchi
    • 1
  • K. Ozaki
    • 1
  • T. Hoshi
    • 2
  1. 1.Dept. of Environmental Biochemistry and ToxicologySchool of Pharmaceutical Sciences, University of ShizuokaShizuoka CityJapan
  2. 2.Laboratory of PhysiologySchool of Food and Nutritional Sciences, University of ShizuokaShizuoka CityJapan

Personalised recommendations