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Research in Experimental Medicine

, Volume 183, Issue 3, pp 167–175 | Cite as

Hepatic pharmacokinetics of glutaraldehyde-treated methotrexate-loaded carrier erythrocytes in dogs

  • J. R. DeLoach
  • C. H. Tangner
  • C. Barton
Original Papers

Summary

Methotrexate-loaded glutaraldehyde-treated canine carrier erythrocytes were used to deliver a 1.6-mg/kg dosage of drug. About 90% of the drug-loaded cells disappeared from circulation within 1 h. Approximately 11 mg of drug reached the liver, and a substantial portion of the methotrexate entered the enterohepatic bile salt circulation. Biochemical tests of liver function, such as alkaline phosphatase and serum glutamine pyruvate transaminase, indicated mild hepatocellular necrosis which was attributed to the action of methotrexate on the hepatocytes. Bilirubin levels were unchanged during and following drug treatment. The plasma half-life of the drug was extended 2.4-fold in the first 24 h following injection.

Key words

Glutaraldehyde Methotrexate Carrier erythrocytes Dog 

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Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • J. R. DeLoach
    • 1
    • 3
  • C. H. Tangner
    • 2
  • C. Barton
    • 2
  1. 1.US Department of AgricultureUS Livestock Insects Lab.KerrvilleUSA
  2. 2.Small Animal Medicine and Surgery, College of Veterinary MedicineTexas A M UniversityCollege StationUSA
  3. 3.Veterinary Toxicology and Entomology Research LaboratoryCollege StationUSA

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