Skip to main content
SpringerLink
Log in

Liver blood flow measured by indocyanine green in conscious unrestrained miniature pigs

  • Original Papers
  • Published:
Research in Experimental Medicine

Summary

For studies in physiology and biochemistry of the liver the measurement of hepatic blood flow is a fundamental precondition. Therefore, the least invasive method, the measurement of liver blood flow by indocyanine green (ICG) dye elimination, was re-evaluated for the miniature pig, an animal model of increasing interest. The accuracy of spectrophotometric determination of ICG was enhanced by addition of 5% of desoxycholic acid. It was shown that ICG was eliminated exclusively by the liver. An extraction rate of 30%–50% was found in mini-pigs, but an insufficient rate of 5%–10% in pigs of German landrace. A liver blood flow ranging from 70 to 160 ml/100g·min−1 was measured. These values are in good accordance with datas from simultaneous estimation of blood flow by133Xenon washout. As shown for a starvation/refeeding cycle, liver blood flow measurement by ICG combined with permanently implanted catheters is a practicable tool for studies of the metabolic and pharmacologic fate of substances resorbed from the gut.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Bradley SE, Ingelfinger FJ, Bradley GT, Curry JJ (1945) The estimation of hepatic blood flow in man. J Clin Invest 24:890–897

    Google Scholar 

  2. Cherrick GR, Stein SW, Leevy CM, Davidson SC (1960) Indocyanine green: observations on its physical properties, plasma decay, and hepatic extraction. J Clin Invest 39:592–600

    PubMed  Google Scholar 

  3. Cobb LA, Barnes SC (1965) Effects of reducing agents on indocyanine green dye. Am Heart J 70:145–146

    PubMed  Google Scholar 

  4. Daneshmend TK, Jackson L, Roberts CJC (1981) Physiological and pharmacological variability in estimated hepatic blood flow in man. Br J Clin Pharmacol 11:491–496

    PubMed  Google Scholar 

  5. Feely J, Wilkinson GR, Wood AJJ (1981) Reduction of liver blood flow and propranolol metabolism by cimetidine. N Engl J Med 304:692–695

    PubMed  Google Scholar 

  6. Fox IJ, Brooker LGS, Heseltine D, Essex HE, Wood EH (1957) A tricarbocyanine dye for continuous recording of dilution curves in whole blood independent of variations in blood saturation. Proc Mayo Clin 32:478–481

    PubMed  Google Scholar 

  7. Hickman R, Saunders SJ, Terblanche J (1974) Liver function in the pig. Total hepatic and portal flow values in vivo. S Afr Med J 48:1197–1200

    PubMed  Google Scholar 

  8. Keiding S (1976) Hepatic elimination kinetics: the influence of hepatic blood flow on clearance determinations. Scand J Clin Lab Invest 36:113–118

    PubMed  Google Scholar 

  9. Ketterer SG, Wiegand BD, Rapaport E (1960) Hepatic uptake and biliary excretion of indocyanine green and its use in estimation of hepatic blood flow in dogs. Am J Physiol 199:481–484

    PubMed  Google Scholar 

  10. Krarup N, Larsen JA (1972) The effect of slight hypothermia on liver function as measured by the elimination rate of ethanol, the hepatic uptake and excretion of indocyanine green and bile formation. Acta Physiol Scand 82:396–407

    Google Scholar 

  11. Leevy CM, Mendenhall CL, Lesko W, Maseo MH (1962) Estimation of hepatic blood flow with indocyanine green. J Clin Invest 41:1169–1179

    PubMed  Google Scholar 

  12. Nielsen NC (1963) Correction for blank density in spectrophotometric dye determination in turbid plasma within the spectral range 600–920 nanometers. Scand J Clin Lab Invest 15:610–612

    PubMed  Google Scholar 

  13. Paschen U, Müller MJ, Seitz HJ (1982) Evaluation of133Xe washout kinetics by controlled pig liver perfusion in vivo. J Surg Res 32:586–597

    PubMed  Google Scholar 

  14. Paschen U, Müller MJ (1986) Serial blood sampling from portal and hepatic vein in conscious unrestrained miniature pigs. Res Exp Med (Berl) 186:87–92

    Google Scholar 

  15. Paumgartner G, Probst P, Kraines R, Leevy CM (1970) Kinetics of indocyanine green removal from the blood. Ann NY Acad Sci 170:134–147

    Google Scholar 

  16. Polack DS, Sufian S, Matsumoto T (1979) Indocyanine green clearance in critically ill patients. Surg Gyn Obstet 149:852–854

    Google Scholar 

  17. Rowell LB, Blackmon JR, Bruce RA (1964) Indocyanine green clearance and estimated hepatic blood flow during mild to maximal exercise in upright man. J Clin Invest 43:1677–1690

    PubMed  Google Scholar 

  18. Shoemaker WC, Steenburg RW, Smith LL, Moore FD (1961) Experimental evaluation of an indicator-dilution technique for estimation of hepatic blood flow. J Lab Clin Med 57:661–669

    Google Scholar 

  19. Stekiel WJ, Kampine JP, Banaszak EF, Smith JJ (1960) Hepatic clearance of indocyanine in the dog. Am J Physiol 198:881–885

    PubMed  Google Scholar 

  20. Teranaka M, Schenk WG (1976) Hepatic blood flow measurement. A comparison of the indocyanine green and electromagnetic techniques in normal and abnormal flow states in the dog. Ann Surg 185:58–63

    Google Scholar 

  21. Wheeler HO, Cranston WJ, Meltzer JJ (1958) Hepatic uptake and biliary excretion of indocyanine green in the dog. Proc Soc Exp Biol NY 99:11–14

    Google Scholar 

  22. Winkler K, Tygstrup N (1960) Determination of hepatic blood flow in man by cardiogreen. Scand J Clin Lab Invest 12:353–361

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. 1. Chirurgische Abt. des AK Altona, Paul-Ehrlich-Str. 1, D-2000, Hamburg 50, Federal Republic of Germany

    U. Paschen

  2. Institut für Physiologische Chemie, Abt. für Biochemische Endokrinologie, Universitäts-Krankenhaus Eppendorf, Universität Hamburg, Federal Republic of Germany

    M. J. Müller

Authors
  1. U. Paschen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. J. Müller
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Paschen, U., Müller, M.J. Liver blood flow measured by indocyanine green in conscious unrestrained miniature pigs. Res. Exp. Med. 187, 71–79 (1987). https://doi.org/10.1007/BF01854971

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01854971

Key words

Search

Navigation