Summary
The effect of acetaldehyde on primary hemostasis and platelet aggregation was studied in vivo and in vitro in the rat. Acetaldehyde was found in circulation following ethanol intoxication or was present after direct i.v. injection. In vitro, acetaldehyde was added to whole blood or platelet plasma suspension. Bleeding time and blood loss were increased upon ethanol intoxication and immediately after i.v. infusion of acetaldehyde. The intrinsic coagulation system, assayed by APT-time, was unaffected. In vivo, ADP and collagen-induced platelet aggregation was inhibited upon the presence of acetaldehyde. In vitro, inhibition of platelet aggregation was observed when acetaldehyde was added to blood, while the addition of the compound to plasma did not affect platelet function.
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References
Barnett AH, Gonzalez-Auvert C, Pyke DA, Saunders TB, Williams R, Dickerson CI, Rauling MD (1981) Blood concentrations of acetaldehyde during chlorpropamide-flush. Br Med J 283:939–942
Bengmark S, Göransson G, Idvall I, Zoucas E (1980) Increased bleeding during liver resection in ethanol intoxicated rats. Thromb Hem 43(3):203–207
Bengmark S, Elmer O, Göransson G, Zoucas E (1981) In vitro effect of ethanol on ADP and collagen induced platelet aggregation. Thromb Haemost 46:673–675
Born BVR (1962) Quantitative investigation into the aggregation of blood platelets. J Physiol (Lond) 162:67–73
Droitte La P, Lamboeuf Y, De Saint Blanquat G (1983) Comparison of rat blood preparation methods for acetaldehyde assay. Ann Biochem 135:180–185
Elmér O, Göransson G, Bengmark S (1977) Ethanol-induced microaggregate formation in pig and rabbit blood. An in vitro study. Thromb Diath Haemorrh 37:344–350
Elmér O, Göransson G, Zoucas E, Bengmark S (1983) Effects of ethanol on platelet aggregation. An in vitro study. Res Exp Med (Berl) 182:13–19
Elmér O, Göransson G, Zoucas E (1984) Impairment of primary hemostasis and platelet function after alcohol ingestion in man. Haemostasis 14:223–228
Eriksson CIP (1980) Problems and pitfalls in acetaldehyde determinations. Alcoholism Clin Exp Res 4:22–29
Haut M, Cowan DH (1974) The effect of ethanol on hemostatic properties of human blood platelets. Am J Med 56:22–30
Inoue K, Komura S, Fukunaga M, Yamasawa K (1983) Oxidation and uptake of acetaldehyde by intact human erythrocytes. Biochem Biophys Acta 759:264–269
Kampen EI van, Ziljstra WC (1961) Standardization of haemoglobinometry. Clin Chim Acta 6:538–541
Kukielka E (1984) The effect of ethanol and acetaldehyde on changes in the haematological indices of the peripheral blood of Wistar rats. Folia Biol (Krakow) 32:109–118
Palmer KR, Jenkins WJ (1982) Impaired acetaldehyde oxidation in alcoholics. Gut 23:729–733
Pietruszko R, Vallari RL (1978) Aldehyde dehydrogenase in human blood. FEBS Lett 92:89–91
Stavenow L, Jenstorp P, Öhlin H (1984) Effects of ethanol and acetaldehyde on cultured rabbit aortic myocytes and human platelets in vitro. Eur J Clin Invest 14:242–246
Stuart M (1979) Ethanol-inhibited platelet prostaglandin synthesis in vitro. J Stud Alcohol 40:1–6
Zeller JA, Dayhoff RE, Eurenius K, Russel W, Ledley RS (1984) Aldehyde-induced platelet aggregation. Clin Lab Haematol 6:145–155
Zoucas E, Bergqvist D, Göransson G, Bengmark S (1982) Effect of acute ethanol intoxication on primary haemostasis, coagulation factors, and fibrinolytic activity. Eur Surg Res 14:33–44
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Supported by grants nos. 142, 156 from the Medical Falculty, University of Lund, Lund, Sweden
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Zoucas, E., Bengmark, S. Effect of acetaldehyde on rat platelet aggregation in vivo and in vitro. Res. Exp. Med. 187, 43–48 (1987). https://doi.org/10.1007/BF01854967
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DOI: https://doi.org/10.1007/BF01854967