Summary
A simple, reliable and efficient miniaturized extracorporeal circulation system for the use in small animals, especially for the perfusion of rat hind limbs, has been described. The system consists of a newly devised bubble oxygenator and heat exchanger and of commercially available roller pumps, polyethylene cannulas and silicone tubes. The minimal and maximal priming volume of the entire system is 4.7 and 16.7 ml, respectively. The efficiency of the system is reflected in a high value of oxygen uptake in the range of 0.061 ml O2·min−1·ml−1 blood, a heat transfer coefficient ranging from 0.96 to 0.31 at flow rates between 1 and 20 ml·min−1, a low pulsation amplitude of the roller pump, a constant flow resistance at the arterial cannula, which implies optimal flow conditions, and in a low blood trauma with plasma hemoglobin concentrations of 47.5 ± 5mg dl−1 after 60min of in vitro perfusion.
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References
Baust G (1971) Die Hämodynamik während des extrakorporalen Kreislaufs. Abhandlung moderner Medizin, Bd 2. Johann Ambrosius Barth, Leipzig
Borst HG (1959) Die künstlichen Oxygenatoren. Thoraxchirurgie 6:312–320
Bücherl ES (1957) In vitro-Untersuchungen zur Frage der Blutveränderungen bei Verwendung einer künstlichen Lunge nach dem Gasdispersionsprinzip. Thoraxchirurgie 5:63–70
Clark RE, Mills M (1970) The infant temptrol oxygenator. J Thorac Cardiovasc Surg 60:54–62
De Wall RA, Warden HE, Read RE, Gott VL, Ziegler NR, Varro RL, Lillehei CW (1956) A simple, expendable, artificial oxygenator for open heart surgery. Surg Clin North Am 36:1025–1034
Dickson JA (1977) The effects of hyperthermia in animal tumour systems. In: Rossi-Fanelli A, Cavaliere R, Mondovi B, Moricca G (eds) Resent results in cancer research. 59:43–111
Dungen JJAM van den, Karliczek GF, Brenken U, Homann van der Heide JN, Widevuur CRH (1982) Clinical study of blood trauma during perfusion with membrane and bubble oxygenators. J Thorax Cardiovasc Surg 83:108–116
Euler US von, Heymans C (1932) An oxygenator for perfusion experiments. J Physiol 74:2–3
Galletti PM, Brecher GA (1962) Heart lung bypass. Grune & Stratton, New York
Gimbel NS, Engelberg J (1954) Blood oxygenators: Theory and studies in design. Surgery 35:645–669
Head LR, Coenen JP, Angola E, Noguere C, Mendelsohn D, Kay EB (1960) Operation of the roller pump for extracorporeal circulation. J Thorac Cardiovasc Surg 39:210–220
Ionescu MI, Wooler GH (1976) Current techniques in extracorporeal circulation. Butterworth, London
Keidel WD (1975) Kurzgefaßtes Lehrbuch der Physiologie. Thieme, Stuttgart
Krementz ET, Carter RD, Sutherland CM, Cambell M (1979) The use of regional chemotherapy in the management of malignant melanomas. World J Surg 3:289–304
McBride CM (1974) Sarcomas of the limbs. Results of adjuvant chemotherapy using isolation perfusion. Arch Surg 109:304–308
Peirce EC (1962) A simplified heat exchanger for perfusion hypothermia. Arch Surg 84:77–81
Peirce EC (1969) Extracorporeal circulation for open heart surgery. Thomas, Springfield
Schwerd W (1962) Der rote Blutfarbstoff und seine wichtigsten Derivate. Schmidt-Römhild, Lübeck
Stehlin JS, Ipolyl PD de, Giovanella BC, Gutierrez AE, Anderson RF (1975) Soft tissue sarcomas of the extremity. Multidisciplinary therapy employing hyperthermic perfusion. Am J Surg 130:643–646
Williams KR, Blustein R (1965) Evaluation of a disposable bubble oxygenator. J Thorac Cardiovasc Surg 50:35–39
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Nagel, K., Ghussen, F., Krüger, I. et al. Miniature equipment for the perfusion of rat limbs. Res. Exp. Med. 187, 1–8 (1987). https://doi.org/10.1007/BF01854962
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DOI: https://doi.org/10.1007/BF01854962