Research in Experimental Medicine

, Volume 184, Issue 4, pp 221–226 | Cite as

The effect of relative bowel rest on collagen metabolism and suture holding capacity in the colonic wall

Studies in the Rat
  • P. Blomquist
  • H. Jiborn
  • B. Zederfeldt
Original Papers


In a previous experimental study it was shown that bowel rest with low residue diet led to a reduction of the non-collagenous substances in the intact, unoperated colon, while the collagen content remained unchanged. In the cecum, both the collagen content and the non-collagenous substances were markedly increased.

In the present investigation the effect of relative bowel rest on collagen synthesis in the colonic wall was studied. Further, a possible influence on mechanical properties was evaluated. It was found out that bowel rest led to a marked decrease of collagen and protein synthesis in the colon. In the cecum, there was an unchanged collagen synthesis but an increased protein synthesis. It can be deduced that lysis of collagen is decreased in all parts of the colon. The depression of collagen turnover in the colonic wall was not accompanied by measurable changes in mechanical strength. It remains to be studied whether the observed changes in collagen metabolism do affect colonic healing.

Key words

Colon Collagen synthesis Mechanical strength Low residue diet 


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  1. 1.
    Arabi Y, Dimock F, Burdon DW, Alexander-Williams J, Keighley MRB (1978) Influence of bowel preparation and antimicrobials on colonic microflora. Br J Surg 65:555–559PubMedGoogle Scholar
  2. 2.
    Axelsson CK, Justesen T (1977) Studies of the duodenal and fecal flora in gastrointestinal disorders during treatment with an elemental diet. Gastroenterology 72:397–401PubMedGoogle Scholar
  3. 3.
    Blomquist P, Jiborn H, Zederfeldt B (1984) The effect of relative bowel rest on collagen in the colonic wall. Res Exp Med (in press)Google Scholar
  4. 4.
    Bounous G, Devroede GJ (1974) Effects of an elemental diet on human fecal flora. Gastroenterology 66:210–214PubMedGoogle Scholar
  5. 5.
    Glotzer DJ, Boyle PL, Silen W (1973) Preoperative preparation of the colon with an elemental diet. Surgery 74:703–707PubMedGoogle Scholar
  6. 6.
    Gurry JF, Ellis-Pegler RB (1976) An elemental diet as preoperative preparation of the colon. Br J Surg 63:969–972PubMedGoogle Scholar
  7. 7.
    Halsted WS (1887) Circular suture of the intestine—an experimental study. Am J Med Sci 94:436–461Google Scholar
  8. 8.
    Hares MM, Alexander-Williams J (1982) The effect of bowel preparation on colonic surgery. World J Surg 6:175–181PubMedGoogle Scholar
  9. 9.
    Jiborn H, Ahonen J, Zederfeldt B (1980) Healing of experimental colonic anastomoses. III. Collagen metabolism in the colon after left colon resection. Am J Surg 139:398–405PubMedGoogle Scholar
  10. 10.
    Johnson WC (1974) Oral elemental diet. A new bowel preparation. Arch Surg 108:32–34PubMedGoogle Scholar
  11. 11.
    Juva K, Prockop DJ (1966) Modified procedure for the assay of3H-or14C-labelled hydroxyproline. Anal Biochem 15:77–83PubMedGoogle Scholar
  12. 12.
    Lindstedt S, Prockop DJ (1961) Isotopic studies on urinary hydroxyproline as evidence for rapidly catabolized forms of collagen in the young rat. J Biol Chem 236:1399–1403PubMedGoogle Scholar
  13. 13.
    Lindström CG, Rosengren J-E, Fork F-T (1979) Colon of the rat. An anatomic, histologic and radiographic investigation. Acta Radiol [Diagn] (Stockh) 20:523–536Google Scholar
  14. 14.
    Matheson DM, Arabi Y, Baxter-Smith D, Alexander-Williams J, Keighley MRB (1978) Randomized multicentre trial of oral bowel preparation and antimicrobials for elective colorectal operations. Br J Surg 65:597–600PubMedGoogle Scholar
  15. 15.
    Peacock EE, Jr (1968) Dynamic aspects of collagen biology. II. Degradation and metabolism. J Surg Res 7:481–497Google Scholar
  16. 16.
    Stragand JJ, Hagemann RF (1977) Effect of luminal contents on colonic cell replacement. Am J Physiol 233:208–211Google Scholar
  17. 17.
    Winitz M, Adams RF, Seedman DA, Davis PN, Jayko LG, Hamilton JA (1970) Studies in metabolic nutrition employing chemically defined diets. II. Effects on gut microflora populations. Am J Clin Nutr 23:546–559PubMedGoogle Scholar
  18. 18.
    Zederfeldt B, Jiborn H, Blomquist P (1982) Effects of different suture techniques on healing of experimental colonic anastomosis. In: Thiede A, Hamelmann H (eds) Moderne Nahtmaterialien und Nahttechniken in der Chirurgie. Springer, Berlin Heidelberg New York, pp 110–121Google Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • P. Blomquist
    • 1
  • H. Jiborn
    • 1
  • B. Zederfeldt
    • 1
  1. 1.Depts. of Surgery and Experimental Research, Malmö General HospitalUniversity of LundMalmöSweden

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