Summary
The effect of black gram and black gram trypsin inhibitor on the protein catabolism of male albino mice has been investigated. Group I was given autoclaved black gram (control), Group II raw black gram and Group III the autoclaved black gram incorporated with 1% black gram trypsin inhibitor. Blood as well as urinary urea and creatinine were found to be elevated in Groups II and III. Increased levels of arginase, ornithine transcarbamylase and transaminases were noted in Groups II and III. The results suggested an enhanced catabolism of proteins evoked by the native black gram trypsin inhibitor.
Similar content being viewed by others
References
Das TK, Waterlow JC (1974) The rate of adaptation of urea cycle enzymes, aminotransferases, and glutamic dehydrogenase to changes in dietary protein intake. Br J Nutr 32:353–373
Hayase K, Yokogoshi H, Yoshida A (1980) Effect of dietary proteins and amino acid deficiencies on urinary excretion of nitrogen and the urea synthesizing system in rats. J Nutr 110:1327–1337
Kamalakannan V, Motlag DB (1982) Influence of dietary black gram (Vigna mungo) trypsin inhibitor on the growth rate and nitrogen absorption in male albino mice. Nutr Rep Int 25:113–125
Cenarruzabeitia MN, Santidrian S, Bello J, Larralde J (1979) Effect of raw field bean(Vicia faba) on amino acid degrading enzymes in rats and chicks. Nutr Metab 23:203–210
Sathyamoorthy V, Kamalakannan V, Motlag DB (1981) Influence of dietary raw green gram (Phaseolus aureus, Roxb) and green gram trypsin inhibitors on the activity of certain protein metabolism enzymes in rats. Ann Nutr Metab 25:334–340
Kamalakannan V, Sathyamoorthy V, Motlag DB (1981) Studies on black gram(Vigna mungo) trypsin inhibitor. J Sci Food Agric 32:1172–1176
Natelson S (1961) Urea with diacetyl monoxime. In: Natelson S (ed) Microtechniques of clinical chemistry. Thomas, Springfield, Ill, pp 434–437
Bonsnes RW, Taussky HH (1945) On the colorimetric determination of creatinine by the Jaffe reaction. J Biol Chem 158:581–591
Varley H (1967) Determination of creatinine. In: Varley H (ed) Practical clinical biochemistry. ELBS, New York, pp 199–200
Schimke RT (1962) Adaptive characteristics of urea cycle enzymes in the rat. J Biol Chem 237:459–468
Ohshita M, Takeda H, Kamiyama Y, Ozawa K, Honjo I (1976) A direct method for the estimation of ornithine carbamoyl transferase activity in serum. Clin Chem Acta 67:145–152
Archibald RM (1944) Determination of citrulline and allantoin and demonstration of citrulline in blood plasma. J Biol Chem 156:121–142
Reitman S, Frankel S (1957) A colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminases. Am J Clin Pathol 28:56–63
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Bernstein J, Weatherall M (1952) In: Statistics for medical and other biological students. Williams and Wilkins, Baltimore
Kean EA (1967) The relationship between nutritive value of dietary protein and activity of liver arginase and kidney transamidinase enzymes. Br J Nutr 21:29–36
Sathyamoorthy V, Kamalakannan V, Motlag DB (1982) Effect of dietary green gram trypsin inhibitor and raw green gram meal (Phaseolus aureus, Roxb) on the pancreatic activity in rats. Res Exp Med (Berl) 180:169–178
Hagihira H, Lin ECC, Samiy AH, Wilson TH (1961) Active transport of lysine, ornithine, arginine and cystine by the intestine. Biochem Biophys Res Commun 4:478–481
Robinson JWL (1966) In: Frotscher KGH (ed) Certain aspects of intestinal amino acid absorption. Odenwald, Darmstadt, pp 1
Munck BG (1965) Amino acid transport by the small intestine of the rat. The effect of amino acid preloading on the trans-intestinal amino acid transport by the everted sac preparation. Biochem Biophys Acta 109:142–150
Munck BG, Schultz SG (1969) Lysine transport across isolated rabbit ileum. J Gen Physiol 53:157–182
Kamalakannan V, Sathyamoorthy V, Motlag DB (1984) Purification, characterisation and kinetics of a trypsin inhibitor from black gram(Vigna mungo). J Sci Food Agric 35:199–206
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kamalakannan, V., Sathyamoorthy, A.V. & Motlag, D.B. Influence of black gram(Vigna mungo) trypsin inhibitory fraction on the hepatic protein catabolism in male albino mice. Res. Exp. Med. 184, 85–90 (1984). https://doi.org/10.1007/BF01852355
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01852355